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公开(公告)号:US20060101537A1
公开(公告)日:2006-05-11
申请号:US11158342
申请日:2005-06-22
申请人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
发明人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
CPC分类号: C12N15/8205
摘要: The present invention relates to a method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic E. grandis×E. urophylla cells and therefrom regenerating stably transformed E. grandis×E. urophylla trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants.
摘要翻译: 本发明涉及一种用于转化和选择植物外植体的方法。 转化方法包括在农杆菌诱导物存在下预培养外植体,并将转化的外植体暴露于加速芽发育的芽再生培养基中。 提供了从该转化方法产生的植物。 特别地,获得转基因大蠊的方法。 桉树细胞,从而再生稳定转化的大x x。 提供桉树。 本发明还提供用于选择和再生植物的培养基,方法和质粒。
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公开(公告)号:US20060130185A1
公开(公告)日:2006-06-15
申请号:US10861909
申请日:2004-06-07
申请人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
发明人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
CPC分类号: C12N15/8205
摘要: The present invention relates to a genotype-independent method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic Eucalyptus and pine cells and regenerating stably transformed Eucalyptus and pine trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants, particularly forest trees.
摘要翻译: 本发明涉及用于转化和选择植物外植体的基因型非依赖性方法。 转化方法包括在农杆菌诱导物存在下预培养外植体,并将转化的外植体暴露于加速芽发育的芽再生培养基中。 提供了从该转化方法产生的植物。 特别地,提供了获得转基因桉树和松树细胞并再生稳定转化的桉树和松树的方法。 本发明还提供用于选择和再生植物,特别是林木的培养基,方法和质粒。
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公开(公告)号:US20060101536A1
公开(公告)日:2006-05-11
申请号:US10981742
申请日:2004-11-05
申请人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
发明人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
CPC分类号: C12N15/8205
摘要: The present invention relates to a method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic E. urophylla and E. saligna cells and therefrom regenerating stably transformed E. urophylla and E. saligna trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants.
摘要翻译: 本发明涉及一种用于转化和选择植物外植体的方法。 转化方法包括在农杆菌诱导物存在下预培养外植体,并将转化的外植体暴露于加速芽发育的芽再生培养基中。 提供了从该转化方法产生的植物。 特别地,提供了用于获得转基因桉树和海绵体细胞以及由此再生稳定转化的欧洲桉树和大肠杆菌树的方法。 本发明还提供用于选择和再生植物的培养基,方法和质粒。
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