公开(公告)号：US5527680A

公开(公告)日：1996-06-18

申请号：US404687

申请日：1995-03-15

申请人： Huu M. Tran ,  Diana M. Smith ,  Lois B. Epstein

发明人： Huu M. Tran ,  Diana M. Smith ,  Lois B. Epstein

IPC分类号： G01N27/447 ,  C12Q1/00 ,  B01D57/02 ,  B01D61/42 ,  C25B7/00

CPC分类号： G01N27/44717

摘要： A flow-through receptacle is disclosed, one end of which is designed to hold multiple plugs of gel material, containing solutes such as macromolecules, excised from electrophoretic separations, and the other end of which is capable of insertion into a recipient matrix that lies between the plates of a slab gel enclosure in an electrophoresis apparatus. The receptacle is used to place the gel plugs, containing the macromolecules or solutes, into electrophoretic contact with a recipient matrix and allows for electrophoretic transfer of the solutes from the plugs into a single concentrated zone in the recipient matrix. The concentrated macromolecules or solutes can then be further processed in the recipient gel or excised and used for procedures requiring greater amounts and concentrations of the solute than are available in the original plugs.

摘要翻译： 公开了一种流通容器，其一端被设计成容纳凝胶材料的多个塞子，其包含从电泳分离物切下的溶质如大分子，并且其另一端能够插入位于 电泳装置中的平板凝胶封套的板。 容器用于将含有大分子或溶质的凝胶塞与受体基质电泳接触，并允许将溶质从栓塞电泳转移到受体基质中的单个浓缩区域中。 然后可以将浓缩的大分子或溶质在接收器凝胶中进一步处理或切除，并用于需要比原始插塞中可获得更大量和浓度的溶质的步骤。