公开(公告)号：US09296806B2

公开(公告)日：2016-03-29

申请号：US12990352

申请日：2009-04-30

Applicant: B Raju Edupuganti ,  Haseeb Jagirdar ,  Manish Kumar ,  Jayaraman Parthipan ,  Vivek Yadav ,  Maharaj K Sahib

Inventor： B Raju Edupuganti ,  Haseeb Jagirdar ,  Manish Kumar ,  Jayaraman Parthipan ,  Vivek Yadav ,  Maharaj K Sahib

IPC: C07K14/62

CPC classification number: C07K14/62

Abstract: The invention provides processes for obtaining a precursor for insulin, analogs or derivatives thereof having correctly bonded cystine bridges. The process involves solubilizing a precursor of insulin, insulin analog or derivatives in an aqueous solution or a buffer containing cysteine or cysteine hydrochloride and one or more of chaotropic auxiliary. The solubilized precursors are refolded by adding diluent to the solubilized mixture (reverse dilution). Further, the solubilized precursors, wherein the concentration of precursor in reaction mixture is more than 0.65 g/liter can also be refolded by diluting the reaction mixture with a diluent optionally comprising about 5-40% v/v of one or more of alcoholic or aprotic solvents.

Abstract translation: 本发明提供了获得具有正确结合的胱氨酸桥的胰岛素前体，其类似物或其衍生物的方法。 该方法包括将胰岛素前体，胰岛素类似物或衍生物溶解在含有半胱氨酸或半胱氨酸盐酸盐的水溶液或缓冲液中，以及一种或多种离液剂。 溶解的前体通过向溶解的混合物中加入稀释剂（反向稀释）来重折叠。 此外，溶解的前体，其中反应混合物中前体的浓度大于0.65g /升也可以通过用任选地包含约5-40％v / v的一种或多种酒精或稀释剂的稀释剂稀释反应混合物来重折叠 非质子溶剂。

公开(公告)号：US20110152507A1

公开(公告)日：2011-06-23

申请号：US12990352

申请日：2009-04-30

Applicant: B Raju Edupuganti ,  Haseeb Jagirdar ,  Manish Kumar ,  Jayaraman Parthipan ,  Vivek Yadav ,  Maharaj K. Sahib

Inventor： B Raju Edupuganti ,  Haseeb Jagirdar ,  Manish Kumar ,  Jayaraman Parthipan ,  Vivek Yadav ,  Maharaj K. Sahib

IPC: C07K14/62

CPC classification number: C07K14/62

Abstract: The invention provides processes for obtaining a precursor for insulin, analogs or derivatives thereof having correctly bonded cystine bridges. The process involves solubilizing a precursor of insulin, insulin analog or derivatives in an aqueous solution or a buffer containing cysteine or cysteine hydrochloride and one or more of chaotropic auxiliary. The solubilized precursors are refolded by adding diluent to the solubilized mixture (reverse dilution). Further, the solubilized precursors, wherein the concentration of precursor in reaction mixture is more than 0.65 g/litre can also be refolded by diluting the reaction mixture with a diluent optionally comprising about 5-40% v/v of one or more of alcoholic or aprotic solvents.

Abstract translation: 本发明提供了获得具有正确结合的胱氨酸桥的胰岛素前体，其类似物或其衍生物的方法。 该方法包括将胰岛素前体，胰岛素类似物或衍生物溶解在含有半胱氨酸或半胱氨酸盐酸盐的水溶液或缓冲液中，以及一种或多种离液剂。 溶解的前体通过向溶解的混合物中加入稀释剂（反向稀释）来重折叠。 此外，溶解的前体，其中反应混合物中前体的浓度大于0.65g /升也可以通过用任选地包含约5-40％v / v的一种或多种酒精或稀释剂的稀释剂稀释反应混合物来重折叠 非质子溶剂。

公开(公告)号：US07608583B2

公开(公告)日：2009-10-27

申请号：US10523999

申请日：2003-11-14

Applicant: Maharaj K Sahib ,  Edupuganti B Raju ,  Umesh S Shaligram

Inventor： Maharaj K Sahib ,  Edupuganti B Raju ,  Umesh S Shaligram

IPC: A61K38/28 ,  C07K1/14

CPC classification number: C07K14/62

Abstract: The present invention relates to the extraction and isolation of insulins from recombinant sources, particularly those expressed in and secreted by yeasts. Organic solvents have been used to extract host surface bound forms of insulin polypeptide. In addition, procedures for combining the steps medium clarification, solvent extraction and chromatography, in order to effect the simultaneous isolation and purification of soluble and membrane bound forms of insulin, is disclosed.

Abstract translation: 本发明涉及从重组来源，特别是由酵母表达和分泌的那些中提取和分离胰岛素。 已经使用有机溶剂来提取宿主表面结合形式的胰岛素多肽。 此外，公开了用于组合培养基澄清，溶剂萃取和色谱法的步骤以实现同时分离和纯化可溶性和膜结合形式的胰岛素的方法。

公开(公告)号：US20080108787A1

公开(公告)日：2008-05-08

申请号：US11597430

申请日：2004-05-24

Applicant: Maharaj K Sahib ,  Edupuganti B Raju ,  Sivaraman Subramaniam ,  Vennapusa R Reddy

Inventor： Maharaj K Sahib ,  Edupuganti B Raju ,  Sivaraman Subramaniam ,  Vennapusa R Reddy

IPC: C07K1/16

CPC classification number: C07K14/62

Abstract: This invention describes processes for purification of insulin or insulin-like material by reverse phase chromatography by using polystyrenic resins as the chromatographic materials. in particular the present invention describes processes for the purification of a particular insulin-like material from chemically and structurally similar contaminants.

Abstract translation: 本发明描述了通过使用聚苯乙烯树脂作为色谱材料通过反相色谱法纯化胰岛素或胰岛素样物质​​的方法。 特别地，本发明描述了从化学和结构相似的污染物纯化特定胰岛素样物质​​的方法。