公开(公告)号：US08187462B2

公开(公告)日：2012-05-29

申请号：US12704732

申请日：2010-02-12

申请人： Simon Olof Harald Bengtsson ,  Alan Gideon Werker ,  Paulo Alexandre da Costa Lemos ,  Maria Ascensao Carvalho Fernandes de Miranda Reis

发明人： Simon Olof Harald Bengtsson ,  Alan Gideon Werker ,  Paulo Alexandre da Costa Lemos ,  Maria Ascensao Carvalho Fernandes de Miranda Reis

IPC分类号： C02F3/30

CPC分类号： C12P7/625 ,  C02F3/30 ,  C02F3/343 ,  C12P39/00

摘要： A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof. Through the consumption of externally supplied VFAs and internally stored glycogen, relatively high levels of PHA in the biomass are produced. Thereafter PHA is separated from the residual biomass.

摘要翻译： 提供了一种用于增加混合培养生物质中PHA生产的方法。 在该方法的第一阶段，与底物相关的有机材料转化成挥发性脂肪酸。 在废水处理方法的情况下，如果废水含有足够的挥发性脂肪酸（VFA）来支持该过程，则不必将有机物质转化为VFA。 在该方法的第二阶段中，利用厌氧 - 好氧选择方法来选择使这些生物体增殖并主导开放混合培养生物质的糖原积累生物体。 通过在选择过程的厌氧处理阶段提供相对高的有机负荷的形式，产生具有相对高水平储存的糖原的糖原积累生物体。 在第三阶段中，在富含糖原的生物体在厌氧或需氧条件下或其组合下进料VFA时，实施PHA积累过程。 通过消耗外部供应的VFAs和内部储存的糖原，生产生物质中相对较高水平的PHA。 此后，PHA与剩余生物量分离。