公开(公告)号：US06551793B1

公开(公告)日：2003-04-22

申请号：US09326806

申请日：1999-06-04

申请人： Alexander Vainstein ,  Michael Vishnevetsky ,  Marianna Ovadis ,  Hanan Itzhaki

发明人： Alexander Vainstein ,  Michael Vishnevetsky ,  Marianna Ovadis ,  Hanan Itzhaki

IPC分类号： C12P2300

CPC分类号： C12N15/8237 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8238 ,  C12N15/825

摘要： A method for production of high amounts of carotenoids in plants, bacteria or cells. Carotenoid-associated proteins CHRC and CHRD isolated from corollas of Cucumis sativus, chrc gene, CHRC deduced sequence, the molecular cloning, cDNA and RNA. A new CHRC promoter able to direct expression for foreign genes. The cloned promoter comprising approximately 3.5 Kb of the CHRC upstream region.

摘要翻译： 用于在植物，细菌或细胞中产生大量类胡萝卜素的方法。 从Cucumis sativus，chrc基因，CHRC推导序列，分子克隆，cDNA和RNA的花冠分离的类胡萝卜素相关蛋白CHRC和CHRD。 一个能够引导外源基因表达的新型CHRC启动子。 克隆的启动子包含约3.5Kb的CHRC上游区。

公开(公告)号：US07129393B1

公开(公告)日：2006-10-31

申请号：US09914146

申请日：2000-02-22

申请人： Alexander Vainstein ,  Amir Zuker ,  Marianna Ovadis

发明人： Alexander Vainstein ,  Amir Zuker ,  Marianna Ovadis

IPC分类号： C12N15/82 ,  C12N15/90 ,  C12N15/84 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N15/8205 ,  C12N15/8207

摘要： A method of transforming a carnation (Dianthus L.) plant genome with a DNA molecule. The method comprises (a) preparing stem explants from carnation cuttings; (b) wounding the explants by microprojectile bombardment; (c) cocultivating the wounded explants with Agrobacterium comprising the DNA molecule under defined conditions of exposure to dark followed by light; (d) excising shoots from the cultivated wounded explants and removing the leaves from the shoots; and (e) culturing the leaves to obtain transgenic shoots transformed with the DNA molecule. Also disclosed are a rolC-transgenic carnation with improved agronomic traits and enhancement of flower fragrance by antisense suppression of the flavonoid gene fht.

摘要翻译： 一种用DNA分子转化康乃馨（石竹L.）植物基因组的方法。 该方法包括（a）从康乃馨切片中制备茎外植体; （b）通过微粒轰击伤害外植体; （c）在暴露于黑暗然后光照的限定条件下，用含有DNA分子的农杆菌共培养受伤的外植体; （d）从栽培的受伤的外植体中切除芽，并从枝条中除去叶子; 和（e）培养叶子以获得用DNA分子转化的转基因芽。 还公开了通过反义抑制类黄酮基因fht，具有改善的农艺性状和增强花香味的rolC-转基因康乃馨。