公开(公告)号：US20090209738A1

公开(公告)日：2009-08-20

申请号：US11665915

申请日：2005-10-21

申请人： Robin William Cranston ,  Jacinta Maree Poole ,  Mark Brendan Hickey

发明人： Robin William Cranston ,  Jacinta Maree Poole ,  Mark Brendan Hickey

IPC分类号： C07K1/14

CPC分类号： C08H1/06 ,  C07K14/4741

摘要： A process for separating keratinous proteins from a keratin-containing material, the process comprising the steps of: subjecting the keratin-containing material to reduction in a liquid medium to solubilise the keratinous proteins under conditions that minimise hydrolysis of the keratinous proteins, to yield a solution of keratinous proteins and undissolved solids; subjecting the solution of keratinous proteins to peroxide oxidation, without any intervening keratin precipitation step; and separating the solution of keratinous proteins from the undissolved solids prior to, at the same time as, or following the oxidation step. Preferred conditions for performing the reduction step involve contacting the keratin-containing material with a solution of an alkali metal sulfide reducing agent at a temperature of between 25 C and 50 C for a time of between 30 and 90 minutes, assuming atmospheric pressure. The peroxide oxidation is suitably carried out within not more than 4 hours after the reduction step, and involves reducing the pH of the solution to a level not less than pH 10, although pH 11.3 is most preferred. The product is demonstrated to have a principal fraction that has a molecular weight above 10 kDa, reflecting that the disulfide bonds in the keratinous proteins are broken without hydrolysis of the proteins.

摘要翻译： 一种从含角蛋白的材料中分离角质蛋白的方法，该方法包括以下步骤：使含角蛋白的物质在液体培养基中减少，以使角质蛋白在最小化角蛋白水解的条件下溶解，得到 角质蛋白和未溶解固体的溶液; 使角质蛋白溶液进行过氧化物氧化，没有任何中间角蛋白沉淀步骤; 以及在氧化步骤之前，之后或之后将角质蛋白溶液与未溶解的固体分离。 进行还原步骤的优选条件包括在25℃至50℃之间的温度下将含角蛋白的物质与碱金属硫化物还原剂的溶液接触，假定为大气压力为30-90分钟。 过氧化物氧化适合在还原步骤后不超过4小时内进行，并且包括将溶液的pH降低至不低于pH 10的水平，尽管最优选为pH 11.3。 该产物被证明具有分子量高于10kDa的主要级分，反映了角质蛋白中的二硫键在不水解蛋白质的情况下被破坏。