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1.发明授权Process for seperating alpha-1-proteinase inhibitor from COHN IV.sub.1 +1V.sub.4 paste 失效从COHN IV1 + 1V4糊剂中分离α-1蛋白酶抑制剂的方法
公开(公告)号：US5616693A
公开(公告)日：1997-04-01
申请号：US673064
申请日：1996-07-01
申请人： Duk S. Hwang , Evelyn Nario , Mark Lepe , Lyndon Luz , Hirokazu Ito , Kazuo Takechi
发明人： Duk S. Hwang , Evelyn Nario , Mark Lepe , Lyndon Luz , Hirokazu Ito , Kazuo Takechi
IPC分类号： C12N9/64 , C07K1/14 , C07K14/775 , C07K14/81
CPC分类号： C07K14/775 , C07K14/8125 , Y10S530/811 , Y10S530/812
摘要： The present invention is directed to a process for purifying alpha-1-PI. The process comprises providing an impure protein fraction which comprises alpha-1-PI. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8.5. PEG is added to precipitate .alpha.-2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl.sub.2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchange medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD.sub.280.
摘要翻译： 本发明涉及纯化α-1-PI的方法。 该方法包括提供包含α-1-PI的不纯蛋白质级分。 将不纯的蛋白质级分悬浮在pH6的水溶液中。回收不溶性蛋白质并将其重悬于pH8.5的水溶液中。 添加PEG以沉淀α-2蛋白。 向包含α-1-PI的PEG上清液中加入ZnCl 2以沉淀粗α-1-PI。 将粗α-1-PI重新溶解并施用于阴离子交换介质。 从阴离子交换介质中回收包含α-1-PI的级分。 通过该方法纯化的α-1-PI具有约1.0单位/ OD280的比活度。
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2.发明授权
公开(公告)号：US5981715A
公开(公告)日：1999-11-09
申请号：US829223
申请日：1997-03-31
申请人： Duk Sung Hwang , Evelyn Nario , Mark Lepe , Lyndon Luz , Hirokazu Ito , Kazuo Takechi
发明人： Duk Sung Hwang , Evelyn Nario , Mark Lepe , Lyndon Luz , Hirokazu Ito , Kazuo Takechi
CPC分类号： C07K14/775 , C07K14/8125 , Y10S530/811 , Y10S530/812
摘要： The present invention is directed to a process for purifying alpha-1-PI. The process comprises providing an impure protein fraction which comprises alpha-1-PI. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8.5. PEG is added to precipitate .alpha.-2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl.sub.2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchange medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD.sub.280.
摘要翻译： 本发明涉及纯化α-1-PI的方法。 该方法包括提供包含α-1-PI的不纯蛋白质级分。 将不纯的蛋白质级分悬浮在pH6的水溶液中。回收不溶性蛋白质并将其重悬于pH8.5的水溶液中。 添加PEG以沉淀α-2蛋白。 向包含α-1-PI的PEG上清液中加入ZnCl 2以沉淀粗α-1-PI。 将粗α-1-PI重新溶解并施用于阴离子交换介质。 从阴离子交换介质中回收包含α-1-PI的级分。 通过该方法纯化的α-1-PI具有约1.0单位/ OD280的比活度。
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