公开(公告)号：US4992372A

公开(公告)日：1991-02-12

申请号：US305311

申请日：1989-02-02

申请人： Alexander R. Pokora ,  Mary M. Stanley ,  William L. Cyrus, Jr.

发明人： Alexander R. Pokora ,  Mary M. Stanley ,  William L. Cyrus, Jr.

IPC分类号： C12N9/08

CPC分类号： C12N9/0065 ,  Y10S435/814 ,  Y10S435/816

摘要： A method for purifying an aqueous peroxidase enzyme containing solution and peroxidase solution purified thereby are disclosed; the method includes the steps of:providing an aqueous solution containing a peroxidase enzyme;adding a water-immiscible or partially water-miscible organic solvent to said aqueous solution to form a mixture having an organic phase and an aqueous phase;agitating said mixture to extract impurities from said aqueous phase into said organic phase;separating said aqueous phase from said organic phase; anddiscarding said organic phase.

摘要翻译： 公开了一种纯化过氧化物酶水溶液和由此提纯过氧化物酶溶液的方法， 该方法包括以下步骤：提供含有过氧化物酶的水溶液; 向所述水溶液中加入与水不混溶或部分水混溶的有机溶剂以形成具有有机相和水相的混合物; 搅拌所述混合物以将杂质从所述水相中提取到所述有机相中; 将所述水相与所述有机相分离; 并丢弃所述有机相。

公开(公告)号：US4507381A

公开(公告)日：1985-03-26

申请号：US598903

申请日：1984-04-10

申请人： Paul C. Adair ,  Homer G. Duda ,  Amy L. Burkholder ,  Richard F. Wright ,  Mary M. Stanley

发明人： Paul C. Adair ,  Homer G. Duda ,  Amy L. Burkholder ,  Richard F. Wright ,  Mary M. Stanley

IPC分类号： G03C8/06 ,  G03C5/54 ,  G03C5/30

CPC分类号： G03C8/06

摘要： A self-contained silver halide diffusion transfer imaging sheet is provided which contains three layers on a substrate. The first layer is a silver halide emulsion formed in a non-ionic material. The intermediate layer is a water barrier. The third layer is a nucleating region which contains collodial silver. A developer, base and silver transport agent can also be located in these layers. For example, the base and silver transport agent can be located in the nucleating layer, with the developer located in the emulsion layer. After the emulsion is exposed to actinic radiation, a non-aqueous activating medium is supplied which initiates the development and diffusion transfer processes. The final result is a positive image in the nucleating layer.

摘要翻译： 提供了独立的卤化银扩散转印成像片，其在衬底上包含三层。 第一层是在非离子材料中形成的卤化银乳剂。 中间层是水屏障。 第三层是含有胶体银的成核区域。 开发商，基地和银运输代理也可以位于这些层。 例如，碱和银输送剂可以位于成核层中，显影剂位于乳剂层中。 在乳液暴露于光化辐射之后，提供非水活化介质，其启动显影和扩散转移过程。 最终结果是成核层中的正像。

公开(公告)号：US4476213A

公开(公告)日：1984-10-09

申请号：US448633

申请日：1982-12-10

申请人： Paul C. Adair ,  Amy L. Burkholder ,  Homer G. Duda ,  Richard F. Wright ,  Mary M. Stanley

发明人： Paul C. Adair ,  Amy L. Burkholder ,  Homer G. Duda ,  Richard F. Wright ,  Mary M. Stanley

IPC分类号： G03C8/06 ,  G03C5/54

CPC分类号： G03C8/06

摘要： A self-contained silver halide diffusion transfer imaging sheet is provided which contains three layers on a substrate. The first layer is a silver halide emulsion formed in a non-ionic material. The intermediate layer is a water barrier. The third layer is a nucleating region which contains colloidal silver. A developer, base and silver transport agent can also be located in these layers. For example, the base and silver transport agent can be located in the nucleating layer, with the developer located in the emulsion layer. After the emulsion is exposed to actinic radiation, a non-aqueous activating medium is supplied which initiates the development and diffusion transfer processes. The final result is a positive image in the nucleating layer.

摘要翻译： 提供了独立的卤化银扩散转印成像片，其在衬底上包含三层。 第一层是在非离子材料中形成的卤化银乳剂。 中间层是水屏障。 第三层是含有胶体银的成核区域。 开发商，基地和银运输代理也可以位于这些层。 例如，碱和银输送剂可以位于成核层中，显影剂位于乳剂层中。 在乳液暴露于光化辐射之后，提供非水活化介质，其启动显影和扩散转移过程。 最终结果是成核层中的正像。