公开(公告)号：US20050008828A1

公开(公告)日：2005-01-13

申请号：US10626472

申请日：2003-07-24

Applicant: Matthew Libera ,  Svetlana Sukhishvili ,  Peter Krsko

Inventor： Matthew Libera ,  Svetlana Sukhishvili ,  Peter Krsko

IPC: C40B40/10 ,  G03F7/20 ,  G03F7/40 ,  B05D3/00 ,  G21H1/00

CPC classification number: B82Y30/00 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00725 ,  C40B40/10 ,  G03F7/2059 ,  G03F7/40 ,  Y10T428/24802

Abstract: Surface-patterned microgels are formed by treating polymer films like electron-beam photoresists, but without destroying or removing the patterned microgels from their substrate. Focused electron beams are used to create patterned microgels on surfaces where the enhanced spatial resolution can be exploited to create gels with characteristic length scales relevant to cellular and sub-cellular processes. Varying the beam intensity allows control of the concentration of proteins that adhere to the resulting microgel. The process can be used to precisely locate the adhesive junction between cells and a substrate and to confine cell growth within defined areas.

Abstract translation: 表面图案的微凝胶通过处理聚合物膜如电子束光致抗蚀剂而形成，但不破坏或从其底物去除图案化的微凝胶。 聚焦电子束用于在表面上产生图案化的微凝胶，其中可以利用增强的空间分辨率来产生具有与细胞和亚细胞过程相关的特征长度尺度的凝胶。 改变光束强度可以控制粘附到所得微凝胶上的蛋白质的浓度。 该方法可用于精确定位细胞和底物之间的粘合剂连接并限制细胞生长在限定区域内。

公开(公告)号：US20060014003A1

公开(公告)日：2006-01-19

申请号：US11141339

申请日：2005-05-31

Applicant: Matthew Libera ,  Svetlana Sukhishvili ,  Peter Krsko ,  Ye Hong

Inventor： Matthew Libera ,  Svetlana Sukhishvili ,  Peter Krsko ,  Ye Hong

IPC: C12M3/00

CPC classification number: A61K47/6903 ,  Y10T428/24802

Abstract: Nanometer-scale hydrogels are formed from a polymer film by exposing said film to a focused electron beam of 1 to 10 nm diameter. The hydrogels may be formed in regular patterns, such as arrays, or in irregular patterns. The hydrogels have a plurality of functional groups that can form covalent bonds with proteins while preserving the natural functionality of the proteins. Such functionalized nanohydrogels may serve as a substrate for attachment of other proteins or cells, or may be used in other biological applications.

Abstract translation: 通过将所述膜暴露于1至10nm直径的聚焦电子束，由聚合物膜形成纳米级水凝胶。 水凝胶可以以规则的图案形成，例如阵列，或以不规则图案形成。 水凝胶具有多个可与蛋白形成共价键的官能团，同时保留蛋白质的天然功能。 这样的官能化纳米水凝胶可以用作其它蛋白质或细胞附着的底物，或者可以用于其它生物学应用。