公开(公告)号：US20050235376A1

公开(公告)日：2005-10-20

申请号：US10514513

申请日：2003-05-12

申请人： Wolf-Bernd Frommer ,  Mechthild Tegeder ,  Axel Hirner ,  Wolfgang Koch

发明人： Wolf-Bernd Frommer ,  Mechthild Tegeder ,  Axel Hirner ,  Wolfgang Koch

IPC分类号： C07K14/415 ,  C12N15/82 ,  C07H21/04 ,  A01H1/00

CPC分类号： C12N15/8251 ,  C07K14/415 ,  C12N15/8243 ,  C12N15/8245

摘要： The invention relates to a method for producing a transgenic plant in which the capability for incorporating undesired nitrogen compounds, especially amino acids, in the storage organs is reduced compared with the wild type, comprising the steps: introducing at least one DNA sequence and/or at least one RNA sequence corresponding to the DNA sequence and/or a mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence with the coding region for an amino acid transporter or parts thereof into a plant cell, wherein the DNA and/or RNA sequence and/or mixed sequence is used in sense or antisense orientation and the expression of an endogenous amino acid transporter gene is prevented or reduced, and regenerating a plant from this plant cell, wherein the DNA and/or RNA sequence is a sequence from Beta vulgaris.

摘要翻译： 本发明涉及一种用于生产转基因植物的方法，其中与野生型相比，在储存器官中掺入不需要的氮化合物，特别是氨基酸的能力降低，包括以下步骤：引入至少一个DNA序列和/或 对应于DNA序列的至少一个RNA序列和/或由对应于具有氨基酸转运蛋白或其部分的编码区的DNA序列的DNA和RNA核苷酸组成的混合序列进入植物细胞，其中所述DNA和/或 RNA序列和/或混合序列以正义或反义方向使用，并且内源氨基酸转运蛋白基因的表达被预防或减少，并且从该植物细胞再生植物，其中所述DNA和/或RNA序列是来自 Beta寻常型