公开(公告)号：US20070292902A1

公开(公告)日：2007-12-20

申请号：US11880311

申请日：2007-07-20

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US20060154315A1

公开(公告)日：2006-07-13

申请号：US11366761

申请日：2006-03-02

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/554 ,  G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两个或更少提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US20050277163A1

公开(公告)日：2005-12-15

申请号：US11154274

申请日：2005-06-15

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/554 ,  G01N33/558 ,  G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。