公开(公告)号：US06268153B1

公开(公告)日：2001-07-31

申请号：US09581493

申请日：2000-06-14

申请人： Michelle Lizotte-Waniewski ,  Steven A. Williams

发明人： Michelle Lizotte-Waniewski ,  Steven A. Williams

IPC分类号： C12Q168

CPC分类号： C12Q1/6888 ,  C12Q1/686

摘要： The present invention discloses polymerase chain reaction (PCR) based assays for the presence of the heartworm parasite Dirofilaria immitis prevalent in dogs and cats, as well as in mosquitoes, which are the transmission vector. The assays are extremely sensitive, and can be used effectively to screen large numbers of samples. The invention provides primers and kits for use in the PCR assay of the invention which are oligonucleotides or oligonucleotide derivatives specific for D. immitis. The invention further discloses methods of detecting the presence of D. immitis in a sample based on PCR which employs the primers of the invention. The invention also discloses methods for rapidly obtaining parasite DNA from blood samples and mosquitoes. The samples that may be assayed include blood samples from mammals (especially dogs and/or cats) suspected of harboring D. immitis and mosquitoes suspected of transmitting the pathogen.

摘要翻译： 本发明公开了基于聚合酶链反应（PCR）的测定方法，用于存在犬和猫中普遍存在的心丝虫寄生虫，以及作为传播载体的蚊子。 该测定非常敏感，可以有效地用于筛选大量样品。 本发明提供了用于本发明的PCR测定中的引物和试剂盒，其是针对小孢子虫特异性的寡核苷酸或寡核苷酸衍生物。 本发明还公开了使用本发明的引物的基于PCR的样品中检测霉菌的存在的方法。 本发明还公开了从血液样品和蚊子中快速获得寄生虫DNA的方法。 可以测定的样品包括怀疑携带怀疑携带病原体的伊蚊和蚊子的哺乳动物（特别是狗和/或猫）的血样。