公开(公告)号：US07348158B2

公开(公告)日：2008-03-25

申请号：US10502959

申请日：2003-01-30

申请人： Hiroshi Matsui ,  Motoko Ohta

发明人： Hiroshi Matsui ,  Motoko Ohta

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60

摘要： A method for specifically quantifying HDL cholesterol in which cholesterol in lipoproteins other than HDL is erased in the first step, and HDL cholesterol is specifically quantified in the second step, by which accurate values can be obtained even in measurements of abnormal samples such as disorder of lipid metabolism and lipoprotein abnormality, is disclosed. The method for quantifying cholesterol in high density lipoprotein according to the present invention comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein by treating a test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein and removing generated hydrogen peroxide; and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of said first step and quantifying hydrogen peroxide generated from cholesterol in high density lipoprotein by actions of cholesterol esterase and cholesterol oxidase. As the cholesterol oxidase used in the first step, one having a molecular weight of not more than 60 kilodaltons is used.

摘要翻译： 用于特异性定量HDL胆固醇的方法，其中在第一步中擦除除HDL之外的脂蛋白中的胆固醇，并且在第二步中特异性地定量HDL胆固醇，即使在异常样品的测量中也可获得精确的值， 脂质代谢和脂蛋白异常。 根据本发明的用于定量高密度脂蛋白中的胆固醇的方法包括通过在不存在以高密度作用的表面活性剂的情况下用胆固醇酯酶和胆固醇氧化酶处理测试样品来清除除了高密度脂蛋白之外的脂蛋白中的胆固醇的第一步骤 脂蛋白和去除产生的过氧化氢; 以及第二步骤，通过胆固醇酯酶和胆固醇氧化酶的作用，将特异性作用于高密度脂蛋白的表面活性剂加入到所述第一步骤的产物中并定量由高密度脂蛋白中的胆固醇产生的过氧化氢。 作为第一步中使用的胆固醇氧化酶，使用分子量不超过60千道尔顿的那些。

公开(公告)号：US20050095658A1

公开(公告)日：2005-05-05

申请号：US10502959

申请日：2003-01-30

申请人： Hiroshi Matsui ,  Motoko Ohta

发明人： Hiroshi Matsui ,  Motoko Ohta

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60

摘要： A method for specifically quantifying HDL cholesterol in which cholesterol in lipoproteins other than HDL is erased in the first step, and HDL cholesterol is specifically quantified in the second step, by which accurate values can be obtained even in measurements of abnormal samples such as disorder of lipid metabolism and lipoprotein abnormality, is disclosed. The method for quantifying cholesterol in high density lipoprotein according to the present invention comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein by treating a test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein and removing generated hydrogen peroxide; and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of said first step and quantifying hydrogen peroxide generated from cholesterol in high density lipoprotein by actions of cholesterol esterase and cholesterol oxidase. As the cholesterol oxidase used in the first step, one having a molecular weight of not more than 60 kilodaltons is used.

摘要翻译： 用于特异性定量HDL胆固醇的方法，其中在第一步中擦除除HDL之外的脂蛋白中的胆固醇，并且在第二步中特异性地定量HDL胆固醇，即使在异常样品的测量中也可获得精确的值， 脂质代谢和脂蛋白异常。 根据本发明的用于定量高密度脂蛋白中的胆固醇的方法包括通过在不存在以高密度作用的表面活性剂的情况下用胆固醇酯酶和胆固醇氧化酶处理测试样品来消除除了高密度脂蛋白之外的脂蛋白中的胆固醇的第一步骤 脂蛋白和去除产生的过氧化氢; 以及第二步骤，通过胆固醇酯酶和胆固醇氧化酶的作用，将特异性作用于高密度脂蛋白的表面活性剂加入到所述第一步骤的产物中并定量由高密度脂蛋白中的胆固醇产生的过氧化氢。 作为第一步中使用的胆固醇氧化酶，使用分子量不超过60千道尔顿的那些。

公开(公告)号：US08765394B2

公开(公告)日：2014-07-01

申请号：US12023899

申请日：2008-01-31

申请人： Hiroshi Matsui ,  Motoko Ohta

发明人： Hiroshi Matsui ,  Motoko Ohta

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60

摘要： A method for specifically quantifying HDL cholesterol in which cholesterol in lipoproteins other than HDL is erased in the first step, and HDL cholesterol is specifically quantified in the second step, by which accurate values can be obtained even in measurements of abnormal samples such as disorder of lipid metabolism and lipoprotein abnormality, is disclosed. The method for quantifying cholesterol in high density lipoprotein according to the present invention comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein by treating a test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein and removing generated hydrogen peroxide; and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of said first step and quantifying hydrogen peroxide generated from cholesterol in high density lipoprotein by actions of cholesterol esterase and cholesterol oxidase. As the cholesterol oxidase used in the first step, one having a molecular weight of not more than 60 kilodaltons is used.

摘要翻译： 用于特异性定量HDL胆固醇的方法，其中在第一步中擦除除HDL之外的脂蛋白中的胆固醇，并且在第二步中特异性地定量HDL胆固醇，即使在异常样品的测量中也可获得精确的值， 脂质代谢和脂蛋白异常。 根据本发明的用于定量高密度脂蛋白中的胆固醇的方法包括通过在不存在以高密度作用的表面活性剂的情况下用胆固醇酯酶和胆固醇氧化酶处理测试样品来清除除了高密度脂蛋白之外的脂蛋白中的胆固醇的第一步骤 脂蛋白和去除产生的过氧化氢; 以及第二步骤，通过胆固醇酯酶和胆固醇氧化酶的作用，将特异性作用于高密度脂蛋白的表面活性剂加入到所述第一步骤的产物中并定量由高密度脂蛋白中的胆固醇产生的过氧化氢。 作为第一步中使用的胆固醇氧化酶，使用分子量不超过60千道尔顿的那些。

公开(公告)号：US20080124748A1

公开(公告)日：2008-05-29

申请号：US12023899

申请日：2008-01-31

申请人： Hiroshi MATSUI ,  Motoko Ohta

发明人： Hiroshi MATSUI ,  Motoko Ohta

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60

摘要： A method for specifically quantifying HDL cholesterol in which cholesterol in lipoproteins other than HDL is erased in the first step, and HDL cholesterol is specifically quantified in the second step, by which accurate values can be obtained even in measurements of abnormal samples such as disorder of lipid metabolism and lipoprotein abnormality, is disclosed. The method for quantifying cholesterol in high density lipoprotein according to the present invention comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein by treating a test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein and removing generated hydrogen peroxide; and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of said first step and quantifying hydrogen peroxide generated from cholesterol in high density lipoprotein by actions of cholesterol esterase and cholesterol oxidase. As the cholesterol oxidase used in the first step, one having a molecular weight of not more than 60 kilodaltons is used.

摘要翻译： 用于特异性定量HDL胆固醇的方法，其中在第一步中擦除除HDL之外的脂蛋白中的胆固醇，并且在第二步中特异性地定量HDL胆固醇，即使在异常样品的测量中也可获得精确的值， 脂质代谢和脂蛋白异常。 根据本发明的用于定量高密度脂蛋白中的胆固醇的方法包括通过在不存在以高密度作用的表面活性剂的情况下用胆固醇酯酶和胆固醇氧化酶处理测试样品来消除除了高密度脂蛋白之外的脂蛋白中的胆固醇的第一步骤 脂蛋白和去除产生的过氧化氢; 以及第二步骤，通过胆固醇酯酶和胆固醇氧化酶的作用，将特异性作用于高密度脂蛋白的表面活性剂加入到所述第一步骤的产物中并定量由高密度脂蛋白中的胆固醇产生的过氧化氢。 作为第一步中使用的胆固醇氧化酶，使用分子量不超过60千道尔顿的那些。