公开(公告)号：US20080233630A1

公开(公告)日：2008-09-25

申请号：US10585860

申请日：2006-03-23

申请人： Young-Ho Kim ,  Il-Joo Shim ,  Myeong-Se Jung ,  Byeong-Gil Lee

发明人： Young-Ho Kim ,  Il-Joo Shim ,  Myeong-Se Jung ,  Byeong-Gil Lee

IPC分类号： C12N1/00 ,  C12M1/42

CPC分类号： B03C1/035

摘要： According to the conventional process of separating necessary cells from a cell mixture containing specific cells tagged with magnetic beads, there is a problem in that separation process is complicated when the separation efficiency is high whereas the separation efficiency is relatively low when the separation process is simplified. The present invention provides an apparatus and method for separating cells from a cell mixture simply and efficiently through the processes of creating a cell mixture layer between upper and lower plates by adjusting a gap between the upper and lower plates and of adjusting a thickness of the created layer and separating the layer while applying a magnetic field to the layer from the upper plate, wherein the lower plate is provided with a cell mixture holding portion, in which the cell mixture containing specific cells tagged with magnetic beads is accommodated in an upwardly convex shape, at a top surface thereof and the upper plate is positioned above the lower plate to face each other and to adsorb the cell mixture accommodated in the cell mixture holding portion of the lower plate into a bottom surface thereof.

摘要翻译： 根据从含有用磁珠标记的特定细胞的细胞混合物中分离所需细胞的常规方法，存在分离过程复杂且分离效率高的问题，而分离过程简化时分离效率相对较低 。 本发明提供了一种用于通过调节上板和下板之间的间隙并调节所产生的厚度的方法，通过在上板和下板之间产生细胞混合层的过程简单有效地分离细胞与细胞混合物的装置和方法 层，并且在从上板向层施加磁场的同时分离层，其中下板设置有电池混合物保持部分，其中含有标记有磁珠的特定电池的电池混合物容纳在向上凸起的形状 在其上表面，并且上板位于下板上方彼此面对并将容纳在下板的电池混合物保持部分中的电池混合物吸附到其底表面中。