Helicase-dependent amplification of nucleic acids
    1.
    发明申请
    Helicase-dependent amplification of nucleic acids 有权
    核酸的螺旋酶依赖性扩增

    公开(公告)号:US20060154286A1

    公开(公告)日:2006-07-13

    申请号:US11327175

    申请日:2006-01-05

    IPC分类号: C12Q1/68 C12P19/34

    CPC分类号: C12P19/34 C12N15/1096

    摘要: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.

    摘要翻译: 提供了用于选择性和指数扩增核酸的方法和试剂盒,并且包括在不存在单链结合蛋白和DNA聚合酶的情况下使用单链解旋酶制剂或热稳定解旋酶,使得可以等温进行扩增。

    Helicase-dependent amplification of RNA
    5.
    发明授权
    Helicase-dependent amplification of RNA 有权
    RNA的螺旋酶依赖性扩增

    公开(公告)号:US07662594B2

    公开(公告)日:2010-02-16

    申请号:US11327175

    申请日:2006-01-05

    IPC分类号: C12P19/34 C07H21/04 C12N9/00

    CPC分类号: C12P19/34 C12N15/1096

    摘要: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.

    摘要翻译: 提供了用于选择性和指数扩增核酸的方法和试剂盒,并且包括在不存在单链结合蛋白和DNA聚合酶的情况下使用单链解旋酶制剂或热稳定解旋酶,使得可以等温进行扩增。