公开(公告)号：US20220295782A1

公开(公告)日：2022-09-22

申请号：US17639565

申请日：2020-09-02

Applicant: NICHIREI BIOSCIENCE INC. ,  RIKEN

Inventor： Ayaka INADA ,  Junko HOSOI ,  Masayo TAKAHASHI ,  Naoshi KOIDE

IPC: A01N1/02 ,  C12N11/02 ,  C12N5/079

Abstract: The present disclosure provides a method for producing a frozen body of a three-dimensional tissue aggregate of retinal pigment epithelial cells, the method including a step of preparing the three-dimensional tissue aggregate of the retinal pigment epithelial cells, and a step of subjecting the three-dimensional tissue aggregate of the retinal pigment epithelial cells to a freezing treatment at a predetermined freezing temperature set in advance using, as an index, the occurrence rate of a linear aggregate of dead cells in the three-dimensional tissue aggregate of the retinal pigment epithelial cells after thawing, thus obtaining the frozen body.