公开(公告)号：US06228632B1

公开(公告)日：2001-05-08

申请号：US09011540

申请日：1998-04-20

申请人： Erwin Schuster ,  Bruno Sproessler ,  Kornelia Titze ,  Michael Gottschalk ,  Nguyen Quoc Khanh ,  Sabine Wolf ,  Hermann Plainer

发明人： Erwin Schuster ,  Bruno Sproessler ,  Kornelia Titze ,  Michael Gottschalk ,  Nguyen Quoc Khanh ,  Sabine Wolf ,  Hermann Plainer

IPC分类号： C12N948

CPC分类号： C12N9/48

摘要： This invention relates to a recombinant deoxyribonucleic acid (DNA) which can be isolated from Aspergillus soyae, characterised in that it codes for a leucine aminopeptidase (LAP) and comprises a nucleotide sequence corresponding to the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP or to a nucleotide sequence derived therefrom which hybridises under stringent conditions with the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP. The invention further relates to vectors and transformed host organisms, and also relates to methods of producing LAP. Enzyme products for the production of protein hydrolysates, as well as protein hydrolysates which are produced correspondingly, also form part of the invention.

摘要翻译： 本发明涉及可从大豆粉中分离的重组脱氧核糖核酸（DNA），其特征在于其编码亮氨酸氨基肽酶（LAP），其包含对应于SEQ ID NO：1中给出的核苷酸序列的核苷酸序列，用于 成熟LAP或衍生自其的核苷酸序列，其在严格条件下与成熟LAP的SEQ ID NO：1中给出的核苷酸序列杂交。 本发明进一步涉及载体和转化的宿主生物，并且还涉及产生LAP的方法。 用于生产蛋白质水解产物的酶产物以及相应生产的蛋白质水解产物也构成本发明的一部分。