公开(公告)号：US4024042A

公开(公告)日：1977-05-17

申请号：US667864

申请日：1976-03-17

申请人： Sven Olof Enfors ,  Nils Ludvig Molin ,  Klaus Hermann Mosbach ,  Hans Jorgen Nilsson

发明人： Sven Olof Enfors ,  Nils Ludvig Molin ,  Klaus Hermann Mosbach ,  Hans Jorgen Nilsson

IPC分类号： C12Q1/00 ,  G01N27/46 ,  C12K1/04

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The invention relates to an enzyme electrode which can be sterilized and regenerated and which has a sensitive surface and an enzyme in contact therewith. A semipermeable membrane is arranged at a distance from the sensitive surface of the detecting electrode and defines a chamber containing the enzyme which is supplied to and discharged from the chamber through a supply conduit and a discharge conduit, respectively. A sterilization device for the enzyme is arranged in the supply conduit for the enzyme. The enzyme electrode is produced by means of the detecting electrode being sterilized in a manner known per se and by means of the enzyme being led into a chamber via a sterilization device through a supply conduit for contact with the sensitive surface of the detecting electrode and for contact with the semipermeable membrane arranged at a distance from said sensitive surface. The enzyme is continuously or intermittently supplied through the supply conduit simultaneously as previously supplied enzyme is discharged from the chamber through the discharge conduit.

摘要翻译： 本发明涉及可以灭菌和再生并且具有敏感表面和与之接触的酶的酶电极。 半透膜设置在与检测电极的敏感表面相距一定距离处，并且限定了含有酶的室，其分别通过供应管道和排出管道供应到室并从室排出。 用于酶的消毒装置设置在用于酶的供应管道中。 酶电极通过以本身已知的方式进行灭菌的检测电极产生，并且通过经由灭菌装置的酶被引导到室中，通过供应导管与检测电极的敏感表面接触，并且 与设置在距离所述敏感表面一定距离处的半透膜接触。 酶通过供应管道同时连续或间歇地供应，因为先前供应的酶通过排出导管从室排出。