公开(公告)号：US20050208671A1

公开(公告)日：2005-09-22

申请号：US11019189

申请日：2004-12-23

申请人： Nobuko Takano ,  Kouzoh Sanchika

发明人： Nobuko Takano ,  Kouzoh Sanchika

IPC分类号： G01N30/86 ,  G01N33/00

CPC分类号： G01N30/8624 ,  G01N30/8631

摘要： An object the invention is to provide a method of analyzing the activation ratio of terminals of a polyoxyalkylene derivative so that the ratio can be accurately measured at a high precision even when the polyoxyalkylene derivative has a high molecular weight. The activation ratio of terminal of a polyoxyalkylene derivative having a terminal active group capable of bonding with a biologically active substance having a molecular weight of 1000 to 100000 is analyzed. The active group is labeled using a labeling reagent having an ionic functional group. The polyoxyalkylene derivative is then analyzed by means of liquid chromatography using an ion-exchange column and an RI detector outputting a chromatogram. The activation ratio of terminal is obtained based on percentage of an area of a peak corresponding to the active group in the chromatogram.

摘要翻译： 本发明的目的是提供一种分析聚氧亚烷基衍生物的末端的活化比例的方法，即使聚氧亚烷基衍生物具有高分子量也能以高精度精确测定该比例。 分析具有能够与分子量为1000〜100000的生物活性物质结合的末端活性基团的聚氧化烯衍生物的末端的活化率。 使用具有离子官能团的标记试剂对活性基团进行标记。 然后使用离子交换柱和输出色谱图的RI检测器通过液相色谱分析聚氧化烯衍生物。 基于色谱图中与活性基团对应的峰面积的百分比获得末端的活化比例。