公开(公告)号：US5667973A

公开(公告)日：1997-09-16

申请号：US474363

申请日：1995-06-07

Applicant: Stanley Fields ,  Ok-Kyu Song

Inventor： Stanley Fields ,  Ok-Kyu Song

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12Q1/6897 ,  C12N15/1055

Abstract: A method is provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

Abstract translation: 提供了一种用于通过重组转录激活物的活性在体内检测第一测试蛋白和第二测试蛋白之间的相互作用的方法。 这种重建利用表达杂合蛋白的嵌合基因。 制备两种类型的杂交蛋白。 第一个杂交体包含与第一个测试蛋白融合的转录激活物的DNA结合结构域。 第二种杂合蛋白含有与第二种测试蛋白融合的转录激活结构域。 如果两个测试蛋白能够相互作用，则它们会使转录激活子的两个结构域紧密相连。 这种接近度足以引起转录，其可以通过含有DNA结合结构域的结合位点的标记基因的活性来检测。

公开(公告)号：US5283173A

公开(公告)日：1994-02-01

申请号：US469285

申请日：1990-01-24

Applicant: Stanley Fields ,  Ok-Kyu Song

Inventor： Stanley Fields ,  Ok-Kyu Song

IPC: C12N15/10 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6897 ,  C12N15/1055

Abstract: Methods are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

Abstract translation: 提供了用于在体内通过重组转录激活物的活性来检测第一测试蛋白和第二测试蛋白之间的相互作用的方法。 这种重建利用表达杂合蛋白的嵌合基因。 制备两种类型的杂交蛋白。 第一个杂交体包含与第一个测试蛋白融合的转录激活物的DNA结合结构域。 第二种杂合蛋白含有与第二种测试蛋白融合的转录激活结构域。 如果两个测试蛋白能够相互作用，则它们会使转录激活子的两个结构域紧密相连。 这种接近度足以引起转录，其可以通过含有DNA结合结构域的结合位点的标记基因的活性来检测。

公开(公告)号：US5468614A

公开(公告)日：1995-11-21

申请号：US189910

申请日：1994-02-01

Applicant: Stanley Fields ,  Ok-Kyu Song

Inventor： Stanley Fields ,  Ok-Kyu Song

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12Q1/6897 ,  C12N15/1055

Abstract: A method and kit are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

Abstract translation: 提供了一种方法和试剂盒，用于通过重组转录激活物的活性来体内检测第一测试蛋白和第二测试蛋白之间的相互作用。 这种重建利用表达杂合蛋白的嵌合基因。 制备两种类型的杂交蛋白。 第一个杂交体包含与第一个测试蛋白融合的转录激活物的DNA结合结构域。 第二种杂合蛋白含有与第二种测试蛋白融合的转录激活结构域。 如果两个测试蛋白能够相互作用，则它们会使转录激活子的两个结构域紧密相连。 这种接近度足以引起转录，其可以通过含有DNA结合结构域的结合位点的标记基因的活性来检测。

公开(公告)号：US20060246417A1

公开(公告)日：2006-11-02

申请号：US11118715

申请日：2005-04-30

Applicant: Ok-kyu Song ,  Sung Key Jang ,  Vit Kim ,  Joon Hyun Kim

Inventor： Ok-kyu Song ,  Sung Key Jang ,  Vit Kim ,  Joon Hyun Kim

IPC: C12Q1/00 ,  C12N1/18 ,  C12N5/06

CPC classification number: C12N1/18

Abstract: The present application discloses a polypeptide assay system that includes a non-mammalian cell in a non-mammalian cell culture medium expressing a heterologous polypeptide that is either displayed on its cell surface such that the polypeptide is the predominant polypeptide displayed on the cell surface or the polypeptide is secreted, and a target mammalian cell that includes a reporter construct in a mammalian cell culture medium.

Abstract translation: 本申请公开了一种多肽测定系统，其包括在表达异源多肽的非哺乳动物细胞培养基中的非哺乳动物细胞，其显示在其细胞表面上，使得多肽是显示在细胞表面上的主要多肽或 分泌多肽，以及哺乳动物细胞培养基中包含报道构建体的靶哺乳动物细胞。