公开(公告)号：US06489110B1

公开(公告)日：2002-12-03

申请号：US09600770

申请日：2000-07-21

申请人： Pieter Oudshoorn ,  Paul R. Klatser

发明人： Pieter Oudshoorn ,  Paul R. Klatser

IPC分类号： C12Q168

CPC分类号： C12Q1/689

摘要： The present invention is related to the detection of bacteria, such as Mycobacteria, in human or animal body fluids such as blood, sputum and urine. The present invention provides a method for assessing the viability of bacteria such as Mycobacterium tuberculosis without the need for propagation of the bacteria. The method of the present invention is in particular useful for assessing the viability of Mycobacteria species such as are M. tuberculosis or M. leprae. With the present invention oligonucleotides are provided that can be used as primers and probes for the amplification of bacterial EF-Tu mRNA. The use of the oligonucleotides according to the invention is not limited to any particular amplification technique or any particular modification thereof. It is evident that the oligonucleotides according to the invention find their use in many different nucleic aid amplification techniques and various methods for detecting the presence of (amplified) bacterial EF-Tu mRNA.

摘要翻译： 本发明涉及在人体或动物体液如血液，痰液和尿液中检测细菌如分枝杆菌。 本发明提供了一种用于评估细菌如结核分枝杆菌的活力的方法，而不需要繁殖细菌。 本发明的方法特别可用于评估分枝杆菌属物种如M.tuberculosis或M. leprae的活力。 本发明提供了可用作扩增细菌EF-Tu mRNA的引物和探针的寡核苷酸。 根据本发明的寡核苷酸的使用不限于任何特定的扩增技术或其任何具体的修饰。 很明显，根据本发明的寡核苷酸可用于许多不同的核酸扩增技术和用于检测（扩增的）细菌EF-Tu mRNA的存在的各种方法。