公开(公告)号：US20120108455A1

公开(公告)日：2012-05-03

申请号：US13200666

申请日：2011-09-27

申请人： Lalitha Kodandapani ,  Louis H. Bookbinder ,  Gregory I. Frost ,  Philip Lee Sheridan ,  Harold Michael Shepard ,  Ge Wei ,  Lei Huang

发明人： Lalitha Kodandapani ,  Louis H. Bookbinder ,  Gregory I. Frost ,  Philip Lee Sheridan ,  Harold Michael Shepard ,  Ge Wei ,  Lei Huang

IPC分类号： C40B30/04 ,  G01N21/64 ,  G01N33/53

CPC分类号： G01N33/5011 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/44 ,  C07K2317/34 ,  G01N33/5014 ,  G01N33/5088 ,  G01N33/53 ,  G01N2333/71

摘要： Methods for evolving or selecting or producing therapeutic proteins that exhibit reduced adverse side-effects and the resulting proteins are provided. For example, provided herein is an in vitro assay to identify conditionally active therapeutic proteins that exhibit better activity within one in vivo environment compared to another in vivo environment. The methods include the steps of a) testing the activity of a protein under conditions in which normal or increased activity is desired; b) testing the activity of the protein under conditions in which reduced activity compared to normal is desired; and c) comparing the activity in a) with b) and selecting/identifying a protein that has greater activity in a) compared to b). The selected/identified protein is a conditionally active protein.

摘要翻译： 提供了演变或选择或产生表现出减少的副作用减轻的所得蛋白质的方法。 例如，本文提供了一种体外测定法，用于鉴定在体内环境中与另一体内环境相比表现出更好活性的条件活性治疗性蛋白质。 所述方法包括以下步骤：a）在需要正常或增加活性的条件下测试蛋白质的活性; b）在需要降低活性的条件下测试蛋白质的活性; 和c）比较a）中的活性和b）和选择/鉴定在a）中比b）具有更大活性的蛋白质。 所选择/鉴定的蛋白质是有条件活性蛋白质。

公开(公告)号：US09683985B2

公开(公告)日：2017-06-20

申请号：US13200666

申请日：2011-09-27

申请人： Lalitha Kodandapani ,  Louis H. Bookbinder ,  Gregory I. Frost ,  Philip Lee Sheridan ,  Harold Michael Shepard ,  Ge Wei ,  Lei Huang

发明人： Lalitha Kodandapani ,  Louis H. Bookbinder ,  Gregory I. Frost ,  Philip Lee Sheridan ,  Harold Michael Shepard ,  Ge Wei ,  Lei Huang

IPC分类号： G01N33/50 ,  C07K16/28 ,  C07K16/30 ,  C07K16/44 ,  G01N33/53

CPC分类号： G01N33/5011 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/44 ,  C07K2317/34 ,  G01N33/5014 ,  G01N33/5088 ,  G01N33/53 ,  G01N2333/71

摘要： Methods for evolving or selecting or producing therapeutic proteins that exhibit reduced adverse side-effects and the resulting proteins are provided. For example, provided herein is an in vitro assay to identify conditionally active therapeutic proteins that exhibit better activity within one in vivo environment compared to another in vivo environment. The methods include the steps of a) testing the activity of a protein under conditions in which normal or increased activity is desired; b) testing the activity of the protein under conditions in which reduced activity compared to normal is desired; and c) comparing the activity in a) with b) and selecting/identifying a protein that has greater activity in a) compared to b). The selected/identified protein is a conditionally active protein.