公开(公告)号：US08431698B2

公开(公告)日：2013-04-30

申请号：US12201680

申请日：2008-08-29

申请人： Philip T. Neff ,  Randall B. Murphy ,  Bruce Bryan

发明人： Philip T. Neff ,  Randall B. Murphy ,  Bruce Bryan

IPC分类号： C07D491/00 ,  C07D495/00 ,  C07D497/00

CPC分类号： G01N21/763 ,  C07D487/04 ,  G01N21/6456

摘要： Bioluminescent endoscopy methods and compounds, wherein an anatomical object is examined by means of filling, perfusing, intubating, injecting, or otherwise administering a solution containing a bioluminescent substance or a mixture of luciferin and luciferase which produces bioluminescence, wherein a color or monochrome image of the object is constituted using the images and information based on bioluminescent emitted by the bioluminescent substance. Procedures are demonstrated which allow bioluminescent solutions to be perfused into certain tissue regions, such as but not limited to the common biliary duct, genitourinary tract, gastro-intestinal tract, cardiovascular system and lymphatic system wherein said structures may be conveniently visualized during surgery to avoid damage to these structures. Such images may also be combined with visual light images. Methods of detection of cancer cells using bioluminescence are provided. Preferred embodiments disclosed include membrane permeant coelenterazine analogs.

摘要翻译： 生物发光内窥镜检查方法和化合物，其中通过填充，灌注，插管，注射或以其他方式施用含有生物发光物质或产生生物发光的荧光素和荧光素酶的混合物的溶液来检查解剖学对象，其中， 使用基于由生物发光物质发射的生物发光的图像和信息构成该对象。 证明了允许将生物发光溶液灌注到某些组织区域中的程序，例如但不限于常见的胆管，泌尿生殖道，胃肠道，心血管系统和淋巴系统，其中所述结构可在手术期间方便地可视化以避免 损坏这些结构。 这些图像也可以与视觉光图像组合。 提供了使用生物发光检测癌细胞的方法。 公开的优选实施方案包括膜渗透性肠溶纤维素类似物。

公开(公告)号：US20090081129A1

公开(公告)日：2009-03-26

申请号：US12201680

申请日：2008-08-29

申请人： Philip T. Neff ,  Randall B. Murphy ,  Bruce Bryan

发明人： Philip T. Neff ,  Randall B. Murphy ,  Bruce Bryan

IPC分类号： A61K49/00 ,  C07D487/02 ,  C12Q1/66

CPC分类号： G01N21/763 ,  C07D487/04 ,  G01N21/6456

摘要： Bioluminescent endoscopy methods and compounds, wherein an anatomical object is examined by means of filling, perfusing, intubating, injecting, or otherwise administering a solution containing a bioluminescent substance or a mixture of luciferin and luciferase which produces bioluminescence, wherein a color or monochrome image of the object is constituted using the images and information based on bioluminescent emitted by the bioluminescent substance. Procedures are demonstrated which allow bioluminescent solutions to be perfused into certain tissue regions, such as but not limited to the common biliary duct, genitourinary tract, gastro-intestinal tract, cardiovascular system and lymphatic system wherein said structures may be conveniently visualized during surgery to avoid damage to these structures. Such images may also be combined with visual light images. Methods of detection of cancer cells using bioluminescence are provided. Preferred embodiments disclosed include membrane permeant coelenterazine analogs.

摘要翻译： 生物发光内窥镜检查方法和化合物，其中通过填充，灌注，插管，注射或以其他方式施用含有生物发光物质或产生生物发光的荧光素和荧光素酶的混合物的溶液来检查解剖学对象，其中， 使用基于由生物发光物质发射的生物发光的图像和信息构成该对象。 证明了允许将生物发光溶液灌注到某些组织区域中的程序，例如但不限于常见的胆管，泌尿生殖道，胃肠道，心血管系统和淋巴系统，其中所述结构可在手术期间方便地可视化以避免 损坏这些结构。 这些图像也可以与视觉光图像组合。 提供了使用生物发光检测癌细胞的方法。 公开的优选实施方案包括膜渗透性肠溶纤维素类似物。

公开(公告)号：US20130058872A1

公开(公告)日：2013-03-07

申请号：US13610242

申请日：2012-09-11

申请人： Philip T. Neff ,  Bruce Bryan ,  Randall B. Murphy

发明人： Philip T. Neff ,  Bruce Bryan ,  Randall B. Murphy

IPC分类号： A61K49/00

CPC分类号： G01N21/763 ,  C07D487/04 ,  G01N21/6456

摘要： Bioluminescent endoscopy methods in which bioluminescent generating system components are combined ex vivo immediately prior to injection or infusion into a tissue area of interest to enable bioluminescent viewing of the tissue. The bioluminescent generating system components are combined by simultaneous injection into a Y tube or other in-line mixing chamber receiving fluid inputs from two or more fluid conduits and providing a fluid output to at least one fluid conduit. The mixed components which are the output of such multi lumen mixing system is injected into a hollow viscus organ or otherwise applied to organs and/or tissues of the body.

摘要翻译： 生物发光内窥镜检查方法，其中将生物发光发生系统组分在注射或注射到目的组织区域之前离体组合以使组织的生物发光观察。 生物发光发生系统部件通过同时注射到Y管或其它在线混合室中，从两个或更多个流体导管接收流体输入，并向至少一个流体导管提供流体输出。 作为这种多腔混合系统的输出的混合成分被注射到空心粘液器官中或以其它方式施加到身体的器官和/或组织上。