公开(公告)号：US20070059833A1

公开(公告)日：2007-03-15

申请号：US11470906

申请日：2006-09-07

Applicant: Linhong Li ,  Linda Liu ,  Cornell Allen ,  Rama Shivakumar ,  James Brady

Inventor： Linhong Li ,  Linda Liu ,  Cornell Allen ,  Rama Shivakumar ,  James Brady

IPC: C12N15/87

CPC classification number: C12N15/87 ,  C12N9/22 ,  C12N2501/70

Abstract: The present invention concerns methods and compositions for improving viability and transgene expression in transfected cells. In one embodiment, the present invention provides a method for increasing the viability of a transfected cell, the method comprising: transfecting a cell with a nucleic acid sequence; and contacting the transfected cell with a nuclease in a manner effective to enhance the viability of the transfected cell.

Abstract translation: 本发明涉及用于改善转染细胞中活力和转基因表达的方法和组合物。 在一个实施方案中，本发明提供了用于增加转染细胞的存活力的方法，所述方法包括：用核酸序列转染细胞; 并以有效增强转染细胞活力的方式使转染的细胞与核酸酶接触。