公开(公告)号：US6124091A

公开(公告)日：2000-09-26

申请号：US867230

申请日：1997-05-30

申请人： Raymond A. Petryshyn

发明人： Raymond A. Petryshyn

IPC分类号： A61K38/00 ,  C12N9/12 ,  C12N15/113 ,  C07H71/04 ,  A61K48/00 ,  C12N15/85 ,  C12Q1/68

CPC分类号： C12N15/1137 ,  C12N9/1205 ,  A61K38/00 ,  C12N2310/315

摘要： The present invention provides a partial cDNA corresponding to an RNA containing double stranded regions (R-RNA), which, when transcribed in vitro, gives rise to an RNA transcript that activates PKR. An approximately 226-252 bp nucleotide (nt) sequence responsible for activation of PKR (the activation sequence) has been identified within the cDNA and isolated. Antisense oligonucleotides corresponding to specific portions of the 252 nt cDNA fragment stimulate proliferation of different cells in culture. Various portions of the cDNA or R-RNA may also be used to inhibit cell proliferation in cell cultures.The present invention further provides pharmaceutical compositions comprising the subject nucleic acid fragments and oligonucleotides. Kits which comprise at least one of the subject isolated nucleic acid molecules or oligonucleotides and a pharmaceutically acceptable carrier are also provided.

摘要翻译： 本发明提供了对应于含有双链区域（RNA-RNA）的RNA的部分cDNA，其在体外转录时产生激活PKR的RNA转录物。 已经在cDNA内鉴定了负责激活PKR（活化序列）的约226-252bp的核苷酸（nt）序列并分离。 对应于252nt cDNA片段的特定部分的反义寡核苷酸刺激培养物中不同细胞的增殖。 cDNA或R-RNA的各种部分也可用于抑制细胞培养物中的细胞增殖。 本发明进一步提供包含本发明核酸片段和寡核苷酸的药物组合物。 还提供了包含受试者分离的核酸分子或寡核苷酸中的至少一个的药盒和药学上可接受的载体。