公开(公告)号：US5916562A

公开(公告)日：1999-06-29

申请号：US123245

申请日：1993-09-20

申请人： Robert S. Munson, Jr. ,  Robert Tolan ,  Pele Chong ,  Raafat Fahim ,  Patrick McVerry ,  Michel Klein

发明人： Robert S. Munson, Jr. ,  Robert Tolan ,  Pele Chong ,  Raafat Fahim ,  Patrick McVerry ,  Michel Klein

IPC分类号： A61K39/00 ,  A61P31/04 ,  C07K14/285 ,  A61K39/02

CPC分类号： C07K14/285 ,  A61K39/00

摘要： The nucleotide sequence of the P2 gene and the derived amino acid sequence of the P2 protein of Haemophilus influenzae type b are disclosed and the methods used to determine the same. Also disclosed are the methods used to clone and express the P2 gene as well as the purification protocol for the gene and protein. Also disclosed is the synthesis of peptides corresponding to the N-terminal and C-terminal ends of the P2 protein. Also disclosed is the use of the P2 protein as a protective agent for the disease caused by Haemophilus influenzae type b and the use of the protein as a carrier for conjugation with an oligosaccharide derived from Haemophilus to generate a potentially efficacious vaccine against the disease. Also disclosed is the use of P2 peptide-conjugates as immunising agents.

摘要翻译： 公开了P2型基因的核苷酸序列和流感嗜血杆菌b型的P2蛋白的衍生氨基酸序列，并且用于确定其的方法。 还公开了用于克隆和表达P2基因的方法以及用于基因和蛋白质的纯化方案。 还公开了对应于P2蛋白的N末端和C末端的肽的合成。 还公开了P2蛋白作为由流感嗜血杆菌b型引起的疾病的保护剂的用途，以及使用该蛋白质作为与衍生自嗜血杆菌的寡糖缀合的载体以产生针对该疾病的潜在有效的疫苗。 还公开了使用P2肽缀合物作为免疫剂。