公开(公告)号：US20230111986A1

公开(公告)日：2023-04-13

申请号：US17905345

申请日：2021-03-19

Applicant: Nikolaos KAVALOPOULOS ,  Roderich RÖMHILD ,  Po-Cheng TANG ,  Johan KREUGER ,  Dan ANDERSSON

Inventor： Nikolaos KAVALOPOULOS ,  Roderich RÖMHILD ,  Po-Cheng TANG ,  Johan KREUGER ,  Dan ANDERSSON

IPC: C12Q1/20 ,  C12M1/32

Abstract: A cell population (55) is cultured on a cell culture substrate (50) while agents contained in agent reservoirs (31, 33, 35) at predefined positions in a culture container (10) diffuse through the substrate (50) and form at least partly overlapping concentration gradients in the substrate (50) within combination areas (41, 43, 45) and substantially non-overlapping concentration gradients in the substrate (50) peripheral to an outer boundary of the agent reservoirs (31, 33, 35). Inhibition end points (61, 63, 65) of respective inhibition zones (60, 62, 64) substantially lacking any growth of the cell population (55) peripheral to the outer boundary of the agent reservoirs (31, 33, 35) and growth end points (71, 73, 75) of respective growth zones (70, 72, 74) comprising growth of the cell population (55) within the combination areas (41, 43, 45) are determined and used to determine interaction effects between the agents on the cell population (55).