公开(公告)号：US20240018452A1

公开(公告)日：2024-01-18

申请号：US18026325

申请日：2020-12-30

Applicant: Zhiyuan LI ,  Rongqi HUANG ,  Shuai LI ,  Chao TIAN ,  Zuoxian LIN ,  Na CHENG

Inventor： Zhiyuan LI ,  Rongqi HUANG ,  Shuai LI ,  Chao TIAN ,  Zuoxian LIN ,  Na CHENG

IPC: C12M3/00 ,  C12M1/00

CPC classification number: C12M21/08 ,  C12M23/34 ,  C12N2513/00

Abstract: An chip for integrated tumor cell behavior experiments, which comprises a functional area I, a functional area II, a functional area III, a functional area IV and a functional area V, wherein the functional area I comprises a cell invasion 3D co-culture plate (400) for cell invasion experiments; the functional area II comprises a cell migration culture hole (500) for cell migration experiments; the functional area III comprises a cell proliferation single-cell culture hole (600) for tumor single-cell culture; the functional area IV comprises an angiogenesis 3D co-culture plate (700) for tumor-related angiogenesis experiments; and the functional area V comprises a tumor single-cell culture hole (803), a matrix glue groove (805) and a tumor cell attraction factor hole (801) connected by matrix glue for tumor single-cell migration or invasion experiments. The single-cell culture, micro-fluidic and 3D culture techniques are comprehensively used in the chip, such that the experiment process is obviously simplified, the experiment efficiency is improved, and the obtained experiment result has a higher accuracy and repeatability.