公开(公告)号：US20140294828A1

公开(公告)日：2014-10-02

申请号：US13994106

申请日：2011-01-06

Applicant: Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

Inventor： Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

IPC: C07K16/08

CPC classification number: C07K16/08 ,  A61K39/00 ,  C07K16/1063 ,  C07K2317/92 ,  C07K2318/20 ,  G01N33/56988 ,  Y02A50/53 ,  Y02A50/60

Abstract: The invention provides methods for the production of single-chain Alphabody polypeptides having detectable binding affinity for, or detectable in vitro activity on, a viral protein of interest, which comprising the step of producing a single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein said variegated amino acid residue positions are located at specific positions in one or more of the alpha-helices of the Alphabody or the linker fragment connecting two consecutive alpha-helices of the Alphabody polypeptides. The invention further provides Alphabodies obtainable by the methods of the invention and uses thereof.

Abstract translation: 本发明提供了生产具有对感兴趣的病毒蛋白质具有可检测的结合亲和力或可检测的体外活性的单链阿拉伯糖苷多肽的方法，其包括产生包含至少100个不同序列的单链Alphabody文库的步骤， 序列单链Alphabody多肽，其中所述Alphabody多肽在限定的5至20个杂化氨基酸残基位置中的至少一个中彼此不同，并且其中所述杂色氨基酸残基位置位于一个或多个 的Alphabody的α-螺旋或连接Alphabody多肽的两个连续的α-螺旋的连接物片段。 本发明还提供了可通过本发明的方法获得的阿尔培布本及其用途。

公开(公告)号：US20130261049A1

公开(公告)日：2013-10-03

申请号：US13994102

申请日：2011-12-02

Applicant: Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

Inventor： Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

IPC: C07K14/00

CPC classification number: C07K14/00 ,  C07K16/1027 ,  C07K16/1063 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2318/20

Abstract: Single-chain Alphabodies that comprise an alpha-helical binding region which mediates binding to a first fusion-driving region of a class-1 viral fusion protein and which structurally mimics a second fusion-driving region of said class-1 viral fusion protein, wherein said first and second fusion-driving regions of said class-1 viral fusion protein are regions which interact to drive the fusion between a virus displaying said class-1 viral fusion protein and a target cell.

Abstract translation: 包含α-螺旋结合区的单链抗体，其介导与第1类病毒融合蛋白的第一融合驱动区结合并且结构上模拟所述1类病毒融合蛋白的第二融合驱动区，其中 所述1类病毒融合蛋白的第一和第二融合驱动区域是相互作用以驱动显示所述1类病毒融合蛋白的病毒与靶细胞之间的融合的区域。