公开(公告)号：US20150219632A1

公开(公告)日：2015-08-06

申请号：US14680641

申请日：2015-04-07

申请人： Masahiko Sato ,  Sachiko Sato

发明人： Masahiko Sato ,  Sachiko Sato

IPC分类号： G01N33/50

CPC分类号： G01N33/5091 ,  C12M41/46 ,  C12Q1/02 ,  G01N33/5017 ,  G02B21/367

摘要： A method and apparatus for quantitative identification of distinctive cellular events occurring in a cell population using a non-fluorescence approach. The apparatus and method comprises an image acquisition unit having a Differential Interference Contrast microscope with a camera, a light source, an environmental chamber allowing carrying out cell culture of at least one cell in a cell population; the image acquisition unit acquiring images of the cell population, at predetermined time points; a cell tracker for individually tracking the at least one cell of the cell population in the images; a distinctive cellular event detector for detecting an occurrence of a distinctive cellular event; a report generator; wherein the distinctive cellular event is selected from the group consisting of: tripolar, tetrapolar, quadpolar cell division, cell fusion, cell death, impaired cell division, cell shape alteration, nuclear shape alteration, inner cellular material accumulation, cell enlargement, engulfing, hyper-mobilization, hypo-mobilization and prolonged doubling time.

摘要翻译： 用于使用非荧光方法定量鉴定在细胞群体中发生的特征性细胞事件的方法和装置。 该装置和方法包括：图像获取单元，其具有带有照相机的差分干涉对比显微镜，光源，允许在细胞群中进行至少一个细胞的细胞培养的环境室; 所述图像获取单元在预定时间点获取所述细胞群体的图像; 用于单独跟踪图像中的细胞群体的至少一个细胞的细胞跟踪器; 用于检测特征性细胞事件的发生的特征性细胞事件检测器; 报告生成器 其中特征性细胞事件选自：三极，四极，四极细胞分裂，细胞融合，细胞死亡，细胞分裂受损，细胞形状改变，核形态改变，内细胞物质积累，细胞增殖，吞噬，超 - 动员不足，延长倍增时间。

公开(公告)号：US20180369261A1

公开(公告)日：2018-12-27

申请号：US16045250

申请日：2018-07-25

申请人： Sachiko Sato ,  Masahiko Sato ,  Ann Rancourt

发明人： Sachiko Sato ,  Masahiko Sato ,  Ann Rancourt

IPC分类号： A61K31/7008 ,  A61P21/00

摘要： Methods of preventing or delaying the weakening of as well as improving the strengthening of muscles includes the step of providing an effective amount or dose of N-actylglucosamine (GlcNAc), related saccharides, or combinations thereof both to improve myogenesis.

公开(公告)号：US08940498B2

公开(公告)日：2015-01-27

申请号：US13867270

申请日：2013-04-22

申请人： Masahiko Sato ,  Sachiko Sato

发明人： Masahiko Sato ,  Sachiko Sato

IPC分类号： C12Q1/02

CPC分类号： G01N33/5017 ,  G01N33/5005 ,  G01N33/5011

摘要： The invention relates to the field of medicine. More particularly, described herein are biological markers associated with phenotypical and/or genotypical alterations in a cell and to methods for assessing mammalian cells. Also described are methods for assessing the condition of a population of cells, methods for assessing carcinogenicity of compounds and methods for quantitatively determine the quality of cell populations.

摘要翻译： 本发明涉及医学领域。 更具体地，本文描述的是与细胞中的表型和/或基因型改变相关的生物学标记和用于评估哺乳动物细胞的方法。 还描述了评估细胞群体状况的方法，用于评估化合物致癌性的方法和定量测定细胞群体质量的方法。

公开(公告)号：US20130288293A1

公开(公告)日：2013-10-31

申请号：US13867270

申请日：2013-04-22

申请人： Masahiko Sato ,  Sachiko Sato

发明人： Masahiko Sato ,  Sachiko Sato

IPC分类号： G01N33/50

CPC分类号： G01N33/5017 ,  G01N33/5005 ,  G01N33/5011

摘要： The invention relates to the field of medicine. More particularly, described herein are biological markers associated with phenotypical and/or genotypical alterations in a cell and to methods for assessing mammalian cells. Also described are methods for assessing the condition of a population of cells, methods for assessing carcinogenicity of compounds and methods for quantitatively determine the quality of cell populations.

摘要翻译： 本发明涉及医学领域。 更具体地，本文描述的是与细胞中的表型和/或基因型改变相关的生物学标记和用于评估哺乳动物细胞的方法。 还描述了评估细胞群体状况的方法，用于评估化合物致癌性的方法和定量测定细胞群体质量的方法。

公开(公告)号：US11244459B2

公开(公告)日：2022-02-08

申请号：US16714104

申请日：2019-12-13

申请人： Masahiko Sato ,  Sachiko Sato ,  Ann Rancourt

发明人： Masahiko Sato ,  Sachiko Sato ,  Ann Rancourt

IPC分类号： G06T7/13 ,  G06T7/181 ,  G06T3/40 ,  G06K9/00 ,  G06T7/12 ,  G06T7/246 ,  G06T7/11 ,  G06T7/187

摘要： The present invention is directed to a method for segmentation and tracking of objects recorded in a grayscale video stream and three-dimensional image slices obtained from various devices, including microscopes, video cameras, medical apparatus, and remote sensing devices, using an approach to correct background, segment images using an improved approach, track a segmented area by evaluating surrounding information of the area, analyze data, and create archives of video streams or three-dimensional image slices, segmented areas and the results of tracking.

公开(公告)号：US20130217061A1

公开(公告)日：2013-08-22

申请号：US13880793

申请日：2011-10-07

申请人： Masahiko Sato ,  Sachiko Sato

发明人： Masahiko Sato ,  Sachiko Sato

IPC分类号： C12Q1/02

CPC分类号： G01N33/5091 ,  C12M41/46 ,  C12Q1/02 ,  G01N33/5017 ,  G02B21/367

摘要： An apparatus for quantitative identification of distinctive cellular events occurring in a cell population using a non-fluorescence approach. The apparatus comprises an image acquisition unit having a Differential Interference Contrast microscope with a camera, a light source, an environmental chamber allowing carrying out cell culture of at least one cell in a cell population; the image acquisition unit acquiring images of the cell population, at predetermined time points; a cell tracker for individually tracking the at least one cell of the cell population in the images; a distinctive cellular event detector for detecting an occurrence of a distinctive cellular event; a report generator; wherein the distinctive cellular event is selected from the group consisting of: tripolar, tetrapolar, quadpolar cell division, cell fusion, cell death, impaired cell division, cell shape alteration, nuclear shape alteration, inner cellular material accumulation, cell enlargement, engulfing, hyper-mobilization, hypo-mobilization and prolonged doubling time.

摘要翻译： 用于使用非荧光方法定量鉴定发生在细胞群体中的特异性细胞事件的装置。 该装置包括具有相机的差分干涉对比显微镜的图像获取单元，光源，允许在细胞群体中进行至少一个细胞的细胞培养的环境室; 所述图像获取单元在预定时间点获取所述细胞群体的图像; 用于单独跟踪图像中的细胞群体的至少一个细胞的细胞跟踪器; 用于检测特征性细胞事件的发生的特征性细胞事件检测器; 报告生成器 其中特征性细胞事件选自：三极，四极，四极细胞分裂，细胞融合，细胞死亡，细胞分裂受损，细胞形状改变，核形态改变，内细胞物质积累，细胞增殖，吞噬，超 - 动员不足，延长倍增时间。