公开(公告)号：US06846636B1

公开(公告)日：2005-01-25

申请号：US09700455

申请日：1999-05-13

Applicant: William S. Argraves ,  Samar Hammad ,  Steingrimur Stefansson ,  Bryan Brewer ,  Alan Remaley

Inventor： William S. Argraves ,  Samar Hammad ,  Steingrimur Stefansson ,  Bryan Brewer ,  Alan Remaley

IPC: C07K14/705 ,  G01N33/53 ,  C07K14/00 ,  C12Q1/62 ,  G01N33/566 ,  G01N33/92

CPC classification number: C07K14/705

Abstract: The present invention provides an isolated mammalian receptor which specifically binds a high density lipoprotein holoparticle, comprising a subunit of approximately 45-600 kDa molecular weight and one or more subunits selected from the group consisting of a subunit of approximately 40-50 kDa molecular weight, a subunit of approximately 120 kDa molecular weight and a subunit of approximately 400 kDa molecular weight. In addition, the present invention provides a method of screening a substance for the ability to modulate the HDL holoparticle binding and/or internalization activity of the receptor of this invention, comprising: a) contacting the substance with a cell producing a functional HDL receptor; and b) assaying the cell for a modulation of the HDL holoparticle binding and/or internalization activity of the receptor, whereby a modulation of the HDL holoparticle binding and/or internalization activity of the receptor identifies a substance with the ability to modulate the HDL holoparticle binding and/or internalization activity of the ADL receptor.

Abstract translation: 本发明提供了分离的哺乳动物受体，其特异性结合高密度脂蛋白holoparticle，其包含约45-600kDa分子量的亚基和一个或多个选自约40-50kDa分子量亚基的亚单位， 约120kDa分子量的亚基和约400kDa分子量的亚单位。 此外，本发明提供了筛选物质以调节本发明受体的HDL holodarticle结合和/或内化活性的能力的方法，包括：a）使物质与产生功能性HDL受体的细胞接触; 和b）测定细胞以调节受体的HDL holoparticle结合和/或内化活性，由此受体的HDL holodarticle结合和/或内化活性的调节鉴定具有调节HDL holoparticle的能力的物质 ADL受体的结合和/或内化活性。