公开(公告)号：US20050267296A1

公开(公告)日：2005-12-01

申请号：US10858599

申请日：2004-06-01

申请人： Arup Siddhanta ,  Ramavatar Meena ,  Kamalesh Prasad ,  Bharatkumar Ramavat ,  Pushpito Ghosh ,  Karuppanan Eswaran ,  Sangaiya Thiruppathi ,  Vaibhav Mantri

发明人： Arup Siddhanta ,  Ramavatar Meena ,  Kamalesh Prasad ,  Bharatkumar Ramavat ,  Pushpito Ghosh ,  Karuppanan Eswaran ,  Sangaiya Thiruppathi ,  Vaibhav Mantri

IPC分类号： C08B37/04

CPC分类号： C08B37/0039 ,  C08B37/0003

摘要： The present invention relates to a simple, direct and cost-effective process for the preparation of agarose of high gel strength and low gelling temperature from naturally occurring or cultivated Gracilaria spp. more particularly Gracilaria dura, said process comprising steps of pre-treating the dry seaweed with alkali, rinsing the pre-treated seaweed until the washing shows a pH ranging between 7 and 8, adding water, autoclaving to obtain extractive, treating the extractive with charcoal and Celite to obtain hot extractive, vacuum-filtering the hot extractive over a Celite bed, freezing the filtrate into a mass and thawing the mass, redissolving the mass in water by heating in an autoclave, repeating the freeze-thaw cycle, straining the product to remove thawed liquid and thereafter squeezing to expel residual liquid to the extent possible to obtain agarose, and an agarose thereof.

摘要翻译： 本发明涉及一种简单，直接和经济有效的方法，用于制备天然存在或培养的江ia属的高凝胶强度和低凝胶化温度的琼脂糖。 所述方法包括以下步骤：用碱对干燥的海藻进行预处理，冲洗预处理的海藻直到洗涤显示pH范围在7至8之间，加入水，高压灭菌以获得萃取物，用木炭处理萃取物 和硅藻土以获得热萃取，在硅藻土床上真空过滤热萃取物，将滤液冷冻成块并解质，通过在高压釜中加热将物质重新溶解在水中，重复冻融循环，使产物变形 以除去解冻的液体，然后挤压以尽可能排出残留液体以获得琼脂糖及其琼脂糖。