公开(公告)号：US20230276790A1

公开(公告)日：2023-09-07

申请号：US18098456

申请日：2023-01-18

Applicant: Sanya Nanfan Research Institute of Hainan University ,  Hainan University

Inventor： Xin WEN ,  Jian LUO ,  Jie HUANG ,  Xin FAN ,  Kexin ZHOU ,  Min YANG ,  Weiwei ZHANG

IPC: A01N1/02

CPC classification number: A01N1/0226 ,  A01N1/0284

Abstract: Disclosed are a hypothermic preserving diluent, a hypothermic preserving method of Plectropomus leopardus semen, and an application thereof, belonging to the technical field of artificial breeding of Plectropomus leopardus, where the hypothermic preserving diluent includes the following components in parts by weight: 3.5 parts of sodium chloride, 1.68 parts of sodium bicarbonate, 2 parts of potassium chloride, 2 parts of reduced glutathione, 0.44 parts of glucose, and 10 parts of fetal bovine serum (FBS), and the Plectropomus leopardus semen is diluted with the diluent according to a dilution ratio of 1:9-1:99, and preserved at 0-4 degree Celsius (° C.).