公开(公告)号：US20060051851A1

公开(公告)日：2006-03-09

申请号：US10489716

申请日：2002-09-12

申请人： Sara Kaminaka ,  Kazuyoshio Kaminaka ,  Masaki Hirashima ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Kazuhiko Takahashi

发明人： Sara Kaminaka ,  Kazuyoshio Kaminaka ,  Masaki Hirashima ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Kazuhiko Takahashi

IPC分类号： C12N9/02 ,  C07H21/04 ,  C12P21/06

CPC分类号： C12N9/0065 ,  C07K14/765 ,  C07K2319/00

摘要： A novel selenocysteine-containing protein is provided which is a substantial entity having an enzymatic activity, typically an activity to reduce peroxide phospholipids. There are provided a gene consisting of a coding sequence for a protein not containing selenocysteine in which the codon for selenocysteine is introduced at a desired position and a selenocysteine insertion sequence resided at the 3′-end of said coding sequence and a protein expressed therefrom, typically a selenocysteine-containing protein having an activity to reduce peroxide phospholipids which is prepared by introducing selenocysteine into albumin and as well as a gene encoding said protein. A novel anti-oxidative substance is provided which can be applied for prevention of worsening, prophylaxis or treatment of various diseases associated with oxidative stress.

摘要翻译： 提供了一种新型含硒代半胱氨酸的蛋白质，其是具有酶活性的实质实体，通常是减少过氧化物磷脂的活性。 提供了由不含硒代半胱氨酸的蛋白质的编码序列组成的基因，其中在期望的位置引入硒代半胱氨酸的密码子，并且存在位于所述编码序列的3'端的硒代半胱氨酸插入序列和由其表达的蛋白质， 通常是含有硒代半胱氨酸的蛋白质，其具有通过将硒代半胱氨酸引入白蛋白而制备的过氧化物磷脂的活性，以及​​编码所述蛋白质的基因。 提供了一种新的抗氧化物质，其可用于预防与氧化应激相关的各种疾病的恶化，预防或治疗。