公开(公告)号：US20130305410A1

公开(公告)日：2013-11-14

申请号：US13843447

申请日：2013-03-15

申请人： Andrew Farmer Bent ,  Matthew Hudson ,  Brian Diers ,  Sara Melito ,  David Edward Cook ,  Teresa Hughes ,  Adam Bayless ,  Jianping Wang ,  Tong Geon Lee ,  Xiaoli Guo

发明人： Andrew Farmer Bent ,  Matthew Hudson ,  Brian Diers ,  Sara Melito ,  David Edward Cook ,  Teresa Hughes ,  Adam Bayless ,  Jianping Wang ,  Tong Geon Lee ,  Xiaoli Guo

IPC分类号： C12N15/82

CPC分类号： C12N15/8285 ,  A01H5/10 ,  C07K14/415 ,  Y02A40/164

摘要： Methods of increasing the resistance of plants, in particular soybeans, to nematodes, in particular soybean cyst nematodes, are provided herein. The methods include increasing the expression of Glyma18g02580, Glyma18g02590 and/or Glyma18g2610 in cells of a plant and in particular in root cells of a plant to increase the resistance of the plant and plant cells to nematodes. The methods include increasing the expression using constitutive promoters or by increasing the copy number of the polynucleotides. Constructs for expressing these polypeptides, transgenic cells, transgenic plants and methods of generating the same are also provided. Methods of screening plant cells for resistance or susceptibility to nematodes are also provided.

摘要翻译： 本文提供了将植物，特别是大豆，特别是大豆孢囊线虫的抗性增加的方法。 所述方法包括增加植物细胞中Glyma18g02580，Glyma18g02590和/或Glyma18g2610的表达，特别是在植物的根细胞中增加植物和植物细胞对线虫的抗性。 所述方法包括使用组成型启动子增加表达或增加多核苷酸的拷贝数。 还提供了用于表达这些多肽，转基因细胞，转基因植物和产生其的方法的构建体。 还提供了筛选植物细胞对线虫抗性或易感性的方法。