-
公开(公告)号：US20090297452A1
公开(公告)日：2009-12-03
申请号：US12298908
申请日：2006-04-28
申请人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Matohide Takahashi , Setsuji Ishida
发明人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Matohide Takahashi , Setsuji Ishida
CPC分类号： G01N33/5088 , G01N33/94
摘要： The present invention relates to a method for quantitatively measuring the muscular relaxing activity of a neurotoxin. Specifically, based on an extent of the activity of muscular relaxation of a neurotoxin from bacteria of Clostridium, the present invention relates to a method for quantification of the efficacy (potential and/or diffusion reaction) of a neurotoxin comprising the following steps of: (a) administering a neurotoxin to the hind leg muscle of one of hind legs of a non-human mammal; (b) applying electric stimulus to said non-human mammal; (c) measuring a compound muscle action potential (CMAP) by contraction of said hind leg muscle to which the neurotoxin is administered and/or of the hind leg muscle of the other hind leg to which the neurotoxin is not administered; and (d) taking amplitude data from the compound muscle action potential (CMAP) obtained by the measurement in step (c) and analyzing an extent of a decrease in amplitude to thereby quantify the efficacy of the muscular relaxing activity by the neurotoxin. In contrast to the mouse LD50 currently used as a potential unit of a botulinum toxin which is measurable at a level of only several units, the quantification method of the efficacy of a neurotoxin of the present invention allows for measurement at a level of as low as 0.01 to 1 unit and hence is a method with a high sensitivity, reproducibility and accuracy.
摘要翻译： 本发明涉及一种定量测定神经毒素的肌肉松弛活性的方法。 具体而言，本发明是基于梭菌属细菌的神经毒素的肌肉弛豫活动的程度，本发明涉及一种定量神经毒素的功效（潜在和/或扩散反应）的方法，包括以下步骤：（ a）将神经毒素施用于非人哺乳动物后腿之一的后腿肌肉; （b）对所述非人哺乳动物施加电刺激; （c）通过神经毒素施用的所述后腿肌肉的收缩和/或不施用神经毒素的另一后腿的后腿肌肉测定复合肌肉动作电位（CMAP）; 和（d）从步骤（c）中测定得到的复合肌肉动作电位（CMAP）获取振幅数据，分析振幅减小的程度，由此量化神经毒素对肌肉松弛活性的功效。 与目前用作肉毒杆菌毒素的潜在单位的小鼠LD50相比，其可测量的水平仅为几个单位，本发明的神经毒素的功效的定量方法允许测量水平低至 0.01〜1单位，因此是具有高灵敏度，重现性和准确性的方法。
-
公开(公告)号：US08949033B2
公开(公告)日：2015-02-03
申请号：US12298908
申请日：2006-04-28
申请人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Motohide Takahashi , Setsuji Ishida
发明人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Motohide Takahashi , Setsuji Ishida
CPC分类号： G01N33/5088 , G01N33/94
摘要： The present invention relates to a method for quantitatively measuring the muscular relaxing activity of a neurotoxin. Specifically, based on an extent of the activity of muscular relaxation of a neurotoxin from bacteria of Clostridium, the present invention relates to a method for quantification of the efficacy (potential and/or diffusion reaction) of a neurotoxin comprising the following steps of: (a) administering a neurotoxin to the hind leg muscle of one of hind legs of a non-human mammal; (b) applying electric stimulus to said non-human mammal; (c) measuring a compound muscle action potential (CMAP) by contraction of said hind leg muscle to which the neurotoxin is administered and/or of the hind leg muscle of the other hind leg to which the neurotoxin is not administered; and (d) taking amplitude data from the compound muscle action potential (CMAP) obtained by the measurement in step (c) and analyzing an extent of a decrease in amplitude to thereby quantify the efficacy of the muscular relaxing activity by the neurotoxin. In contrast to the mouse LD50 currently used as a potential unit of a botulinum toxin which is measurable at a level of only several units, the quantification method of the efficacy of a neurotoxin of the present invention allows for measurement at a level of as low as 0.01 to 1 unit and hence is a method with a high sensitivity, reproducibility and accuracy.
摘要翻译： 本发明涉及一种定量测定神经毒素的肌肉松弛活性的方法。 具体而言，本发明是基于梭菌属细菌的神经毒素的肌肉弛豫活动的程度，本发明涉及一种定量神经毒素的功效（潜在和/或扩散反应）的方法，包括以下步骤：（ a）将神经毒素施用于非人哺乳动物后腿之一的后腿肌肉; （b）对所述非人哺乳动物施加电刺激; （c）通过神经毒素施用的所述后腿肌肉的收缩和/或不施用神经毒素的另一后腿的后腿肌肉测定复合肌肉动作电位（CMAP）; 和（d）从步骤（c）中测定得到的复合肌肉动作电位（CMAP）获取振幅数据，分析振幅减小的程度，由此量化神经毒素对肌肉松弛活性的功效。 与目前用作肉毒杆菌毒素的潜在单位的小鼠LD50相比，其可测量的水平仅为几个单位，本发明的神经毒素的功效的定量方法允许测量水平低至 0.01〜1单位，因此是具有高灵敏度，重现性和准确性的方法。
-
公开(公告)号：US08577618B2
公开(公告)日：2013-11-05
申请号：US12298908
申请日：2006-04-28
申请人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Motohide Takahashi , Setsuji Ishida
发明人： Tetsuhiro Harakawa , Hirotoshi Nakano , Yasushi Torii , Sachio Okuda , Ryuji Kaji , Takashi Sakamoto , Motohide Takahashi , Setsuji Ishida
IPC分类号： G01N33/50
摘要： The present invention relates to a method for quantitatively measuring the muscular relaxing activity of a neurotoxin. Specifically, based on an extent of the activity of muscular relaxation of a neurotoxin from bacteria of Clostridium, the present invention relates to a method for quantification of the efficacy (potential and/or diffusion reaction) of a neurotoxin comprising the following steps of: (a) administering a neurotoxin to the hind leg muscle of one of hind legs of a non-human mammal; (b) applying electric stimulus to said non-human mammal; (c) measuring a compound muscle action potential (CMAP) by contraction of said hind leg muscle to which the neurotoxin is administered and/or of the hind leg muscle of the other hind leg to which the neurotoxin is not administered; and (d) taking amplitude data from the compound muscle action potential (CMAP) obtained by the measurement in step (c) and analyzing an extent of a decrease in amplitude to thereby quantify the efficacy of the muscular relaxing activity by the neurotoxin. In contrast to the mouse LD50 currently used as a potential unit of a botulinum toxin which is measurable at a level of only several units, the quantification method of the efficacy of a neurotoxin of the present invention allows for measurement at a level of as low as 0.01 to 1 unit and hence is a method with a high sensitivity, reproducibility and accuracy.
-
-
搜索结果
3 条记录 (耗时 0.01 秒)
