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公开(公告)号：US20120129155A1
公开(公告)日：2012-05-24
申请号：US13186985
申请日：2011-07-20
申请人： Ann Dak-Yee Kwong , James Daniel Frantz , Douglas John Bartels , Chao Lin , Benjamin Shames , Sheila Seepersaud , Judith A. Lippke , Tara Lynn Kieffer , Yi Zhou , Eileen Z. Zhang , James C. Sullivan
发明人： Ann Dak-Yee Kwong , James Daniel Frantz , Douglas John Bartels , Chao Lin , Benjamin Shames , Sheila Seepersaud , Judith A. Lippke , Tara Lynn Kieffer , Yi Zhou , Eileen Z. Zhang , James C. Sullivan
CPC分类号： C12Q1/707 , C12Q1/6883
摘要： A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
摘要翻译： 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段，其中第一阶段PCR使用第一外部引物 和第二外引物，第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO：2所示的核苷酸序列; 或SEQ ID NO：9，其中任选地1,2或3个核苷酸是比SEQ ID NO：9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO：3或4所示的核苷酸序列; 或如SEQ ID NO：10或11所示的核苷酸序列，其中任选1,2或3个核苷酸是与SEQ ID NO：10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO：5所示; 或SEQ ID NO：12，其中任选地1,2或3个核苷酸是与SEQ ID NO：12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO：6或7所示的核苷酸序列 ; 或如SEQ ID NO：13或14所示的核苷酸序列，其中任选地1,2或3个核苷酸是与SEQ ID NO：13和14相同的其它核苷酸。
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公开(公告)号：US08679740B2
公开(公告)日：2014-03-25
申请号：US13186985
申请日：2011-07-20
申请人： Ann Dak-Yee Kwong , James Daniel Frantz , Douglas John Bartels , Chao Lin , Benjamin Shames , Sheila Seepersaud , Judith A. Lippke , Tara Lynn Kieffer , Yi Zhou , Eileen Z. Zhang , James C. Sullivan
发明人： Ann Dak-Yee Kwong , James Daniel Frantz , Douglas John Bartels , Chao Lin , Benjamin Shames , Sheila Seepersaud , Judith A. Lippke , Tara Lynn Kieffer , Yi Zhou , Eileen Z. Zhang , James C. Sullivan
CPC分类号： C12Q1/707 , C12Q1/6883
摘要： A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
摘要翻译： 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段，其中第一阶段PCR使用第一外部引物 和第二外引物，第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO：2所示的核苷酸序列; 或SEQ ID NO：9，其中任选地1,2或3个核苷酸是比SEQ ID NO：9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO：3或4所示的核苷酸序列; 或如SEQ ID NO：10或11所示的核苷酸序列，其中任选1,2或3个核苷酸是与SEQ ID NO：10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO：5所示; 或SEQ ID NO：12，其中任选地1,2或3个核苷酸是与SEQ ID NO：12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO：6或7所示的核苷酸序列 ; 或如SEQ ID NO：13或14所示的核苷酸序列，其中任选地1,2或3个核苷酸是与SEQ ID NO：13和14相同的其它核苷酸。
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