公开(公告)号：US20090105171A1

公开(公告)日：2009-04-23

申请号：US11885639

申请日：2006-03-07

申请人： Noritoshi Nagaya ,  Kunio Miyatake ,  Kenji Kangawa ,  Shinsuke Murakami

发明人： Noritoshi Nagaya ,  Kunio Miyatake ,  Kenji Kangawa ,  Shinsuke Murakami

IPC分类号： A61K31/7088 ,  A61P43/00

CPC分类号： A61K38/22

摘要： [Problems to be Solved] An objective of the present invention is to provide methods for regenerating or repairing damaged tissues using adrenomedullin. Another objective is to provide pharmaceutical agents that comprise adrenomedullin as an active ingredient for regenerating or repairing damaged tissues.[Means for Solving the Problems] To solve the above problems, the present inventors administered adrenomedullin (hereinafter indicated as AM) or physiological saline to C57BL/6 mice, and counted the numbers of mononuclear cells and Sca-1-positive cells in the blood. The result showed that AM increased the numbers of mononuclear cells and stem cell antigen-1-positive cells in the blood. It was also shown that by administering AM to a mouse model of pulmonary emphysema and a rat model of acute myocardial infarction, the quantity of bone marrow-derived cells that migrated and settled into the damaged tissues increased, and the recruited bone marrow cells differentiated into blood vessels, alveoli, and cardiac muscle at the lesion sites. Further, decrease in infarct size, suppression of the enlargement of alveolar diameter, and improvement of organ functions were confirmed in the models of myocardial infarction and pulmonary emphysema.

摘要翻译： [待解决的问题]本发明的目的是提供使用肾上腺髓质素再生或修复受损组织的方法。 另一个目标是提供包含肾上腺髓外素作为活性成分的药剂，用于再生或修复受损组织。 解决问题的手段为了解决上述问题，本发明人对C57BL / 6小鼠施用肾上腺髓质素（以下称为AM）或生理盐水，并计数血液中的单核细胞数和Sca-1阳性细胞数 。 结果表明，AM增加了血液中单核细胞和干细胞抗原-1阳性细胞的数量。 还表明，通过对小鼠肺气肿模型和急性心肌梗死的大鼠模型给予AM，迁移并沉降到受损组织中的骨髓来源的细胞数量增加，并且募集的骨髓细胞分化为 病变部位的血管，肺泡和心肌。 此外，在心肌梗死和肺气肿模型中证实了梗死面积的减少，肺泡直径的增大的抑制和器官功能的改善。