公开(公告)号：US20120156673A1

公开(公告)日：2012-06-21

申请号：US13378497

申请日：2010-06-24

Applicant: Paulo Cezar De Lucca ,  Shujie Dong ,  Robert Jason Christopher Geijskes ,  Erik Martin Dunder ,  Manuel Benito Sainz

Inventor： Paulo Cezar De Lucca ,  Shujie Dong ,  Robert Jason Christopher Geijskes ,  Erik Martin Dunder ,  Manuel Benito Sainz

IPC: C12Q1/68 ,  C12N15/82

CPC classification number: C12N15/8205

Abstract: The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium-inoculated sugar cane tissue or cell thereof; b) co-cultivating said Agrobacterium-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium-inoculated-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.

Abstract translation: 本发明提供了生产转化的甘蔗组织或其细胞的方法，所述方法包括：a）用农杆菌接种悬浮液接种甘蔗组织或其细胞，所述农杆菌包含感兴趣的核酸，以获得农杆菌 接种的甘蔗组织或其细胞; b）在不存在共培养基的情况下，将所述农杆菌接种的甘蔗组织或其细胞共培养一段足以减少所述农杆菌接种的甘蔗组织或其细胞的原始重量的时间段; 和c）选择转化的甘蔗组织或其包含所述感兴趣的核酸的细胞。 本发明的转化方法提供了转基因甘蔗植物的转化频率和恢复的增加。

公开(公告)号：US08952219B2

公开(公告)日：2015-02-10

申请号：US13378497

申请日：2010-06-24

Applicant: Paulo Cezar De Lucca ,  Shujie Dong ,  Robert Jason Christopher Geijskes ,  Erik Martin Dunder ,  Manuel Benito Sainz

Inventor： Paulo Cezar De Lucca ,  Shujie Dong ,  Robert Jason Christopher Geijskes ,  Erik Martin Dunder ,  Manuel Benito Sainz

IPC: A01H1/02 ,  C12N15/82

CPC classification number: C12N15/8205

Abstract: The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium-inoculated sugar cane tissue or cell thereof; b) co-cultivating said Agrobacterium-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.

Abstract translation: 本发明提供了生产转化的甘蔗组织或其细胞的方法，所述方法包括：a）用农杆菌接种悬浮液接种甘蔗组织或其细胞，所述农杆菌包含感兴趣的核酸，以获得农杆菌 接种的甘蔗组织或其细胞; b）在不存在共培养培养基的情况下，将表达所述农杆菌接种的甘蔗组织或其细胞共培养足够时间以减少所述农杆菌接种的甘蔗组织或其细胞的原始重量; 和c）选择转化的甘蔗组织或其包含所述感兴趣的核酸的细胞。 本发明的转化方法提供了转基因甘蔗植物的转化频率和恢复的增加。