公开(公告)号：US20110111400A1

公开(公告)日：2011-05-12

申请号：US12823035

申请日：2010-06-24

申请人： In Gyu KIM ,  Tae Lim Kim ,  Kug Chan Kim ,  Eun Jin Kim ,  So Yong Lee ,  Il Lae Jung

发明人： In Gyu KIM ,  Tae Lim Kim ,  Kug Chan Kim ,  Eun Jin Kim ,  So Yong Lee ,  Il Lae Jung

IPC分类号： C12Q1/68 ,  C12N5/02 ,  G01N33/53

CPC分类号： G01N33/5011 ,  G01N33/574

摘要： Disclosed herein is a method for enhancing sensitivity of cancer cells to compounds or radiation by inhibiting the expression of testis-specific protein, Y-encoded like 5 (TSPYL5). More specifically, because methylation of TSPYL5 protein expressed in lung cancer cell line was inhibited to increase the expression level of the gene, resistance to stress such as radiation or anticancer agents was increased. Because the sensitivity of cancer cells to stress such as radiation or anticancer agents was increased by inhibiting the expression of the TSPYL5 gene to promote the apoptosis of the cells, an anticancer supplement agent containing an inhibitor of the expression or activity of the TSPYL5 gene of the present invention inhibits the growth of cancer cells and enhances the sensitivity to various stresses to maximize the apoptosis. Thus, when used in combination with radiotherapy or chemotherapy, the anticancer supplement agent may be used very usefully for anticancer treatment.

摘要翻译： 本文公开了通过抑制Y编码的5（TSPYL5）的睾丸特异性蛋白质的表达来增强癌细胞对化合物或辐射的敏感性的方法。 更具体地说，由于在肺癌细胞系中表达的TSPYL5蛋白的甲基化被抑制以增加基因的表达水平，所以对抗应激性（如放射线或抗癌剂）的抗性增加。 因为通过抑制TSPYL5基因的表达以促进细胞凋亡来增加癌细胞对诸如放射线或抗癌剂之类的应激的敏感性，所以可以使用含有TSPYL5基因的表达或活性抑制剂的抗癌补充剂 本发明抑制癌细胞的生长并且增强对各种应激的敏感性以使细胞凋亡最大化。 因此，当与放射治疗或化疗组合使用时，抗癌补充剂可以非常有用地用于抗癌治疗。

公开(公告)号：US08343940B2

公开(公告)日：2013-01-01

申请号：US12823035

申请日：2010-06-24

申请人： In Gyu Kim ,  Tae Lim Kim ,  Kug Chan Kim ,  Eun Jin Kim ,  So Yong Lee ,  Il Lae Jung

发明人： In Gyu Kim ,  Tae Lim Kim ,  Kug Chan Kim ,  Eun Jin Kim ,  So Yong Lee ,  Il Lae Jung

IPC分类号： C12N15/11 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N33/5011 ,  G01N33/574

摘要： Disclosed herein is a method for enhancing sensitivity of cancer cells to compounds or radiation by inhibiting the expression of testis-specific protein, Y-encoded like 5 (TSPYL5). More specifically, because methylation of TSPYL5 protein expressed in lung cancer cell line was inhibited to increase the expression level of the gene, resistance to stress such as radiation or anticancer agents was increased. Because the sensitivity of cancer cells to stress such as radiation or anticancer agents was increased by inhibiting the expression of the TSPYL5 gene to promote the apoptosis of the cells, an anticancer supplement agent containing an inhibitor of the expression or activity of the TSPYL5 gene of the present invention inhibits the growth of cancer cells and enhances the sensitivity to various stresses to maximize the apoptosis. Thus, when used in combination with radiotherapy or chemotherapy, the anticancer supplement agent may be used very usefully for anticancer treatment.

摘要翻译： 本文公开了通过抑制Y编码的5（TSPYL5）的睾丸特异性蛋白质的表达来增强癌细胞对化合物或辐射的敏感性的方法。 更具体地说，由于在肺癌细胞系中表达的TSPYL5蛋白的甲基化被抑制以增加基因的表达水平，所以对抗应激性（如放射线或抗癌剂）的抗性增加。 因为通过抑制TSPYL5基因的表达以促进细胞凋亡来增加癌细胞对诸如放射线或抗癌剂之类的应激的敏感性，所以可以使用含有TSPYL5基因的表达或活性抑制剂的抗癌补充剂 本发明抑制癌细胞的生长并且增强对各种应激的敏感性以使细胞凋亡最大化。 因此，当与放射治疗或化疗组合使用时，抗癌补充剂可以非常有用地用于抗癌治疗。