公开(公告)号：US5942407A

公开(公告)日：1999-08-24

申请号：US882594

申请日：1997-06-25

申请人： Lance A. Liotta ,  Bryan C. Christiansen ,  Alan R. Day ,  Tabitha Harlacher ,  Katherine Paweletz

发明人： Lance A. Liotta ,  Bryan C. Christiansen ,  Alan R. Day ,  Tabitha Harlacher ,  Katherine Paweletz

IPC分类号： G01N33/543 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/00 ,  G01N33/53

CPC分类号： G01N33/582 ,  G01N33/54366

摘要： A light generating dry disposable device for determining the presence of analytes in a test sample is disclosed. The device comprises a first zone containing conjugated ligand which is capable of reacting with analytes in the test sample. The ligand is conjugated with a photoprotein or related enzyme. The device further comprises a second trapping zone comprising immobilized analyte. The device also includes a third zone containing a reporter system that activates light generation by the conjugate. The conjugates are maintained in the first zone such that they are removable from the first zone when reacted with the soluble analytes from the test sample passing through the first zone, but not removed from the second trapping zone in the absence of such analytes. The third zone contains material capable of reacting with the photoprotein- or enzyme-linked ligand to produce a light-emitting reaction which indicates the presence of the analyte being tested. The present invention provides dry flow through zones. The trapping zone is juxtaposed with the light activation zone such that only formed analyte conjugate complexes enter the light activation zone. Also disclosed is the use of a luminometer for measuring the amount of light generated from the assay device upon detection of analyte. Other provisions are disclosed for recording and quantifying the amount of analyte, such as for example, photographic film.

摘要翻译： 公开了一种用于确定测试样品中分析物的存在的发光干燥一次性装置。 该装置包括含有共轭配体的第一区，其能够与测试样品中的分析物反应。 配体与光蛋白或相关酶缀合。 该装置还包括包含固定分析物的第二捕集区。 该装置还包括含有报告系统的第三区域，其通过缀合物激活光产生。 当共轭物与来自经过第一区的测试样品的可溶性分析物反应时，它们可以从第一区域移除，但在不存在这种分析物的情况下不从第二捕获区域移除。 第三区含有能够与光蛋白或酶连接的配体反应以产生发光反应的物质，其指示待测分析物的存在。 本发明提供干流穿过区域。 捕获区与光活化区并置，使得仅形成的分析物共轭复合物进入光活化区。 还公开了使用发光计来测量在检测分析物时由测定装置产生的光量。 公开了用于记录和定量分析物的量的其它规定，例如照相胶片。