公开(公告)号：US09046516B2

公开(公告)日：2015-06-02

申请号：US12062170

申请日：2008-04-03

申请人： Hiroshi Tsuchiya ,  Takuya Odawara

发明人： Hiroshi Tsuchiya ,  Takuya Odawara

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/573 ,  G01N33/58

CPC分类号： G01N33/54393 ,  G01N33/573 ,  G01N33/581

摘要： An immunoassay method comprising: forming, on a solid phase, a complex comprising a measurement substance in a sample, an alkaline phosphatase (ALP) labeling substance capable of binding to the measurement substance, and a first binding substance which is capable of binding to the measurement substance by antigen-antibody reaction and is capable of binding to the solid phase; reacting ALP in the ALP-labeling substance bound to the measurement substance in the complex, with a substrate for ALP contained in a substrate solution, in the presence of an auxiliary reagent solution comprising an inhibitor for inhibiting an endogenous ALP; and detecting a reaction product produced by the reaction between ALP and the substrate.

摘要翻译： 一种免疫测定方法，包括：在固相上形成包含样品中的测量物质的复合物，能够结合所述测量物质的碱性磷酸酶（ALP）标记物质，以及能够与所述测定物质结合的第一结合物质 测量物质通过抗原 - 抗体反应并能够结合固相; 在包含用于抑制内源性ALP的抑制剂的辅助试剂溶液的存在下，将与所述复合物中的测量物质结合的ALP标记物质与含有底物溶液中的ALP的底物反应; 并检测通过ALP和基板之间的反应产生的反应产物。

公开(公告)号：US20080254492A1

公开(公告)日：2008-10-16

申请号：US12062170

申请日：2008-04-03

申请人： Hiroshi TSUCHIYA ,  Takuya Odawara

发明人： Hiroshi TSUCHIYA ,  Takuya Odawara

IPC分类号： C12Q1/42

CPC分类号： G01N33/54393 ,  G01N33/573 ,  G01N33/581

摘要： An immunoassay method comprising: forming, on a solid phase, a complex comprising a measurement substance in a sample, an alkaline phosphatase (ALP) labeling substance capable of binding to the measurement substance, and a first binding substance which is capable of binding to the measurement substance by antigen-antibody reaction and is capable of binding to the solid phase; reacting ALP in the ALP-labeling substance bound to the measurement substance in the complex, with a substrate for ALP contained in a substrate solution, in the presence of an auxiliary reagent solution comprising an inhibitor for inhibiting an endogenous ALP; and detecting a reaction product produced by the reaction between ALP and the substrate.

摘要翻译： 一种免疫测定方法，包括：在固相上形成包含样品中的测量物质的复合物，能够结合所述测量物质的碱性磷酸酶（ALP）标记物质，以及能够与所述测定物质结合的第一结合物质 测量物质通过抗原 - 抗体反应并能够结合固相; 在包含用于抑制内源性ALP的抑制剂的辅助试剂溶液的存在下，将与所述复合物中的测量物质结合的ALP标记物质与含有底物溶液中的ALP的底物反应; 并检测通过ALP和基板之间的反应产生的反应产物。