公开(公告)号：US5589625A

公开(公告)日：1996-12-31

申请号：US374229

申请日：1995-01-18

申请人： Mart Saarma ,  Merikke Kelve ,  Erkki Truve ,  Teemu Teeri

发明人： Mart Saarma ,  Merikke Kelve ,  Erkki Truve ,  Teemu Teeri

IPC分类号： C12N9/12 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8283 ,  C12N9/12

摘要： This invention discloses transgenic plants, such as transgenic tobacco and potato, having resistance to multiple viral taxonomic groups using parts of the 2,5A oligoadenylate pathway. In particular, said plants are genetically engineered to contain a DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity. By this means a step in the 2,5A oligoadenylate pathway heretofore believed to be missing in all plants is provided so that viral infection in the transgenic plants is inhibited via a 2,5A dependent endonuclease. Moreover, this invention relates to a process for the production of said transgenic plants by transfection with a genetically engineered DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity.

摘要翻译： 本发明公开了转基因植物，例如转基因烟草和马铃薯，其具有使用部分2,5A寡聚腺苷酸途径的多种病毒分类组的抗性。 特别地，所述植物被遗传工程化以含有编码至少一种具有2,5A合成酶活性的多肽的DNA序列。 通过这意味着提供迄今为止认为在所有植物中缺失的2,5A寡腺苷酸途径中的步骤，使得转基因植物中的病毒感染通过2,5A依赖性内切核酸酶被抑制。 此外，本发明涉及通过用编码具有2,5A合成酶活性的至少一种多肽的遗传工程改造的DNA序列转染来生产所述转基因植物的方法。