公开(公告)号：US5888733A

公开(公告)日：1999-03-30

申请号：US724824

申请日：1996-10-02

申请人： Jens J. Hyldig-Nielsen ,  Karl-Johan Pluzek ,  Tom Just

发明人： Jens J. Hyldig-Nielsen ,  Karl-Johan Pluzek ,  Tom Just

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07K5/00

CPC分类号： C12Q1/6841

摘要： Methodologies for determining the presence of specific nucleic acid sequences in a sample of eucaryotic origin using in situ hybridization are described. The in situ hybridization is performed using a hybridization solution comprising at least one binding partner capable of hybridizing to the specific nucleic acid sequence to be determined so as to form hybrids and a hybrid destabilizing agent in an amount effective to decrease the melting temperature of hybrids formed between the nucleic acid and the binding partner so as to increase the specific binding and decrease the non-specific binding. The binding partner used is a polymeric strand of polymerized moieties having a non-cyclic backbone, the polymeric strand being capable of hybridizing to the nucleic acid sequence to be determined. The method is particularly suitable for diagnosing different human diseases such as bacterial and viral infections, genetic diseases and neoplastic disorders.

摘要翻译： 描述了使用原位杂交确定真核来源样品中特定核酸序列的存在的方法。 原位杂交使用包含至少一个结合配偶体的杂交溶液进行，所述结合配偶体能够与需要测定的特异性核酸序列杂交以形成混合物和混合去稳定剂，其量有效降低形成的杂交体的熔解温度 在核酸和结合配偶体之间，以增加特异性结合并降低非特异性结合。 使用的结合配偶体是具有非环状主链的聚合部分的聚合物链，所述聚合物链能够与待确定的核酸序列杂交。 该方法特别适用于诊断不同的人类疾病，如细菌和病毒感染，遗传疾病和肿瘤性疾病。