公开(公告)号：US20240336913A1

公开(公告)日：2024-10-10

申请号：US18563310

申请日：2022-05-26

申请人： Derek BOGDANOFF ,  Chang KIM ,  Tomasz NOWAKOWSKI ,  The Regents of the University of California ,  CHAN ZUCKERBERG BIOHUB, INC.

发明人： Derek Bogdanoff ,  Chang Kim ,  Tomasz Nowakowski

IPC分类号： C12N15/10

CPC分类号： C12N15/1065

摘要： Provided herein is a method for producing a population of symmetrically barcoded transposomes, i.e., transposomes that are loaded with a pair of identical double-stranded adapters. In some embodiments, this method may comprise amplifying a template that contains a randomized sequence and a transposon end sequence on a solid support by bridge polymerase chain reaction (PCR) to produce uniquely barcoded clusters of single-stranded amplification products that are tethered to the support, processing the single-stranded amplification products so that the transposon end sequence is double-stranded and at the end of the products, and adding transposase to the support under conditions by which the transposase binds to the double-stranded transposon end sequences, to produce population of symmetrically barcoded transposomes.