公开(公告)号：US20080235817A1

公开(公告)日：2008-09-25

申请号：US11987206

申请日：2007-11-28

申请人： Tsuneko Okazaki ,  Masashi Ikeno ,  Toshihide Itou ,  Nobutaka Suzuki

发明人： Tsuneko Okazaki ,  Masashi Ikeno ,  Toshihide Itou ,  Nobutaka Suzuki

IPC分类号： A01K67/027 ,  C07K14/00 ,  C12N5/06

CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K48/00 ,  C12N2800/204 ,  C12N2800/206 ,  C12N2800/208 ,  C12N2820/00 ,  C12N2820/85

摘要： It is intended to provide an artificial mammalian chromosome which is stably held in mammalian cells and allows efficient expression of a target gene carried thereby. Namely, a first cyclic vector containing a mammalian centromere sequence and a selection marker gene and a second cyclic vector containing a functional sequence are transferred into mammalian host cells. Then transformed cells are selected by using the above-described selection marker gene and cells holding an artificial mammalian chromosome are selected from among the transformed cells thus selected. Thus, it is possible to construct an artificial mammalian chromosome which has a mammalian replication origin, the mammalian centromere sequence and the functional sequence, is in a cyclic form, can be replicated in mammalian cells, extrachromosomally held in the host cells and transferred to daughter cells in cell division.

摘要翻译： 旨在提供稳定保持在哺乳动物细胞中的人造哺乳动物染色体，并且能够有效地表达由其携带的靶基因。 即，将含有哺乳动物着丝粒序列和选择标记基因的第一个环状载体和含有功能序列的第二个环状载体转移到哺乳动物宿主细胞中。 然后通过使用上述选择标记基因选择转化的细胞，并且从这样选择的转化细胞中选择携带人造哺乳动物染色体的细胞。 因此，可以构建具有哺乳动物复制起点，哺乳动物着丝粒序列和功能序列为环状的人造哺乳动物染色体，可以在哺乳动物细胞中复制，染色体外保留在宿主细胞中并转移到女儿 细胞分裂细胞。