公开(公告)号：US20130122523A1

公开(公告)日：2013-05-16

申请号：US13635348

申请日：2011-03-21

申请人： Ferdinand Hermann Bahlmann ,  Danilo Fliser ,  Martina Sester ,  Urban Sester

发明人： Ferdinand Hermann Bahlmann ,  Danilo Fliser ,  Martina Sester ,  Urban Sester

IPC分类号： G01N33/68

CPC分类号： G01N33/5695 ,  G01N33/6863 ,  G01N33/6866 ,  G01N33/6869 ,  G01N2333/55 ,  G01N2333/57 ,  G01N2800/56

摘要： An in-vitro process for the quick determination of the infection status of a Mycobacterium tuberculosis infection from whole blood in terms of active or latent tuberculosis, comprising the steps of: stimulating an antigen-specific T cell that is present in a first sample of whole blood with purified protein derivative (PPD) in the presence of antibodies against CD28, or CD28 and CD49d; processing of PPD by antigen-presenting cells (APC) by incubating for 1.5 h to 2.5 h, especially for 2 h, at 35° C. to 39° C., especially at 37° C., optionally with adding CO2; then adding a secretion inhibitor; effecting an intensive mixing; and another incubation for a period of at least 2.5 h at a temperature of from 35° C. to 39° C., and determining a cytokine profile from both the intracellular INF-γ production and the intracellular IL-2 production of the antigen-specific T cell; wherein the presence of an active tuberculosis is indicated by a shift of the cytokine profile towards IFN-γ single positive cells accompanied by a decrease of IFN-γ/IL-2 double positive T cells.

摘要翻译： 一种在活性或潜伏性结核病方面快速测定全血结核分枝杆菌感染感染状态的体外方法，包括以下步骤：刺激存在于全部第一样品中的抗原特异性T细胞 在存在针对CD28或CD28和CD49d的抗体的情况下，用纯化蛋白质衍生物（PPD） 通过在35℃至39℃，特别是37℃，任选加入CO 2的温度下孵育1.5小时至2.5小时，特别是2小时，通过抗原呈递细胞（APC）处理PPD; 然后加入分泌抑制剂; 进行密集混合; 并且在35℃至39℃的温度下另外孵育至少2.5小时的时间，并且从细胞内INF-γ产生和抗原决定簇的细胞内IL-2产生中确定细胞因子谱， 特异性T细胞; 其中活动性结核病的存在通过伴随IFN-γ/ IL-2双重阳性T细胞减少的IFN-γ单个阳性细胞的细胞因子分布的移位来表示。