公开(公告)号：US06194205B1

公开(公告)日：2001-02-27

申请号：US09152653

申请日：1998-09-14

申请人： Martin Staege ,  Bettina Kempkes ,  Georg W. Bornkamm ,  Wolfgang Hammerschmidt ,  Ursula Zimber-Strobl ,  Axel Polack

发明人： Martin Staege ,  Bettina Kempkes ,  Georg W. Bornkamm ,  Wolfgang Hammerschmidt ,  Ursula Zimber-Strobl ,  Axel Polack

IPC分类号： C12N510

CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  C12N2501/52 ,  C12N2502/11 ,  C12N2510/04

摘要： T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desired antigen; and (g) optionally purifying and isolating the stimulated T cells.

摘要翻译： 通过（a）以允许调节这些基因中的至少一个的表达和/或功能的方式将永生化基因引入抗原呈递细胞来刺激具有所需抗原特异性的T细胞，以获得条件永生化的抗原呈递细胞; （b）以表达和/或消除至少一种永生化基因的功能停止后，以允许抗原表达的方式将编码所需抗原的基因导入永生化细胞; （c）通过永生化基因的表达和/或功能活化来扩增永生化的抗原呈递细胞; （d）通过停止表达和/或消除至少一种可控永生化基因的功能来完成永生化抗原呈递细胞的增殖; （e）继续表达抗原; （f）加入包含T细胞的白细胞，培养细胞混合物以刺激针对所需抗原的T细胞; 和（g）任选地纯化和分离受刺激的T细胞。