公开(公告)号：US20070166307A1

公开(公告)日：2007-07-19

申请号：US10561411

申请日：2004-06-18

申请人： Andrew Bushell ,  Kathryn Wood ,  Mahzuz Karim ,  Vanessa Oliveira ,  Brigit Sawitzki

发明人： Andrew Bushell ,  Kathryn Wood ,  Mahzuz Karim ,  Vanessa Oliveira ,  Brigit Sawitzki

IPC分类号： A61K39/395 ,  C12N5/08

CPC分类号： C07K16/2866 ,  A61K39/39541 ,  A61K2039/505 ,  C07K16/2812 ,  A61K2300/00

摘要： The present invention relates to a transplant rejection in an animal suppressed by administration of an antibody directed at a cell surface antigen selected from the group consisting of CD4, CD8, CD154, LFA-1, CD80, CD86 and ICAM-1, preferably an anti-CD4 antibody, together with a non-cellular protein antigen to generate in the animal a population of regulatory T-lymphocytes; reactivating said population of regulatory T-lymphocytes by further administration to the animal of the non-cellular protein antigen; and transplanting said organ or tissue whilst said population of regulatory T-lymphocytes is activated. Regulatory T cells can be generated ex vivo by culturing T cells with an antibody directed at a cell surface antigen selected from the group consisting of CD4, CD8, CD154, LFA-1, CD80, CD86 and ICAM-1, in the presence of cells that present either alloantigen or a non-cellular protein antigen. Ex vivo generated T-lymphocytes can be used as an alternative method of overcoming transplant rejection or in combination with the in vivo method. A similar approach can be adopted for the treatment of autoimmune conditions.

摘要翻译： 本发明涉及通过施用针对选自CD4，CD8，CD154，LFA-1，CD80，CD86和ICAM-1的细胞表面抗原的抗体而抑制的动物中的移植排斥反应，优选抗 - -CD4抗体与非细胞蛋白抗原一起在动物体内产生调节性T淋巴细胞群; 通过进一步施用非细胞蛋白抗原的动物来重新激活所述调节性T淋巴细胞群; 并移植所述器官或组织，同时所述调节性T淋巴细胞群被激活。 通过在细胞存在下用针对选自CD4，CD8，CD154，LFA-1，CD80，CD86和ICAM-1的细胞表面抗原的抗体培养T细胞，可以离体产生调节性T细胞 其呈现同种异体抗原或非细胞蛋白抗原。 可以使用体外产生的T淋巴细胞作为克服移植排斥反应或与体内方法组合的替代方法。 可以采用类似的方法来治疗自身免疫病症。