公开(公告)号：US20120081518A1

公开(公告)日：2012-04-05

申请号：US13244297

申请日：2011-09-24

申请人： Yuan-An Liu ,  Ann-Shyn Chiang ,  Shiue-Cheng Tang

发明人： Yuan-An Liu ,  Ann-Shyn Chiang ,  Shiue-Cheng Tang

IPC分类号： H04N13/02

CPC分类号： G02B21/006

摘要： The present invention discloses a method of visualizing the 3-dimensional microstructure of a thick biological tissue. This method includes: a process of immersing thick, opaque biological tissues in the optical-clearing solution, for example FocusClear (U.S. Pat. No. 6,472,216), and utilizing an optical scanning microscope and a cutter. In microscopy, the cutter removes a portion of the tissue after each round of optical scanning. Each round of optical scanning follows the principal that the depth of the removal plane is less than the depth of the boundary plane derived from the scanning This method acquires an image stack to provide the information of thick biological tissue's 3-dimensional microstructure with minimal interference by the tissue removal.

摘要翻译： 本发明公开了一种可视化厚生物组织的三维微结构的方法。 该方法包括：将厚的不透明的生物组织浸入光学清除溶液中的方法，例如FocusClear（美国专利号6,472,216），并使用光学扫描显微镜和切割器。 在显微术中，切割器在每轮光学扫描后移除一部分组织。 每轮光学扫描遵循的原理是去除平面的深度小于从扫描导出的边界平面的深度。该方法获取图像堆叠，以最小的干扰提供厚生物组织的3维微结构的信息 组织去除。